Catsper3 (NM_001252488) Mouse Untagged Clone

CAT#: MC227163

Catsper3 (untagged) - Mouse cation channel, sperm associated 3 (Catsper3), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Catsper3
• Synonyms: 4921522D01Rik; CACRC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227163 representing NM_001252488
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAACATTTTCACCACAACCCTGTACGAGTCAAGTCGGGCTCACTGTTTGCTACAGCATCGGAAG
  CATTGCAGGCAAGACTGAGCAAGATTAAGAGGAAGGATAAGGAGTGCCAGGCTTACTTCAGGAAGGTTAT
  TAAGAGCACTTTCTTCCAGATTGTGATGATCACCACGGTCACCACCAACTCCTTTTTACTGGTCTTGGGG
  ACTAATTATGACATACAATTCGAGTTTTTCAGAACCTTTGAGGGGAATCATTCTGCATACCTCCACTTTG
  CTGATGGCATCCAGTCTCTACGAATCCTCAAGCTTATCTCCTACAGTAGGGGCATCAGGACACTCATCAT
  CGCTGTGGGGGAGACGGTCTACACTGTGGCCTCGGTGCTGACGCTGCTCTTCCTCCTCATGTTTGTGTTC
  GCGATCCTGGGATTCTGCCTATTTGGCGTGACGGACAGAGGCGACCTGGAGAACTGGGGGAACCTGGCTT
  CAGCTTTCTTTACTCTCTTCAGTTTGGCCACGGTTGATGGCTGGACTGACCTGCAGGAAGAGCTGGACAA
  GAGGAAGTTTACTGTGAGCCGGGCGTTTACTATCCTCTTCATCTTGCTTGCATCCTTCATCTTCCTCAAC
  ATGTTTGTGGGTGTGATGATCATGCACACGGAGGATTCCATGAAAAAGTTTGAGCGGGATCTGACGTTGG
  AGAGGAACCTTGCGATTATGGAGGAGAAGCAAATAATCCTGAAACGCCAGCAAGAGGAGGTCAACAGGCT
  GATGAACACACAGAAAAGTGGTAGCATGAACTTCATTGATATGGTGGAGGGCTTCAAGAAGACCCTGCGG
  CACACAGACCCCATGGTTCTGGATGACTTCAGCACTAGTCTCTCCTTCATTGATATCTACTTGGTCACTC
  TGGACAACCAAGATGTTATTGTCAGCAAGCTTCAGGAGCTCTACTGTGAGATTGTGAACGTGCTGAGCCT
  GATGTTGGAAGACATGCCCAAGGAGAGCTCGTCCAGCCTCTCGGGACTAAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252488
• Insert Size: 1035 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252488.1, NP_001239417.1
• RefSeq Size: 1199 bp
• RefSeq ORF: 1035 bp
• Locus ID: 76856
• Cytogenetics: 13 B1
• Gene Summary: Voltage-gated calcium channel that plays a central role in sperm cell hyperactivation. Controls calcium entry to mediate the hyperactivated motility, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Activated by intracellular alkalinization.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the coding region compared to variant 1. The resulting protein (isoform 3) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.