Brwd1 (NM_001103179) Mouse Untagged Clone

CAT#: MC225248

Brwd1 (untagged) - Mouse bromodomain and WD repeat domain containing 1 (Brwd1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Brwd1
• Synonyms: 5330419I02Rik; D530019K20Rik; G1-403-16; repro5; Wdr9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225248 representing NM_001103179
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCTTCGCCGGCCCGACGTCCGGTGCCTCTCATCGAGTCGGAGCTGTACTTTCTCATCGCCC
  GCTACCTCTCAGCTGGCCCGTGTCGCCGAGCGGCGCAGGTGCTGGTGCAGGAGCTGGAGCAGTACCAGCT
  GTTGCCGAAGAGGTTGGACTGGGAGGGCAACGAGCACAACAGGAGCTACGAGGAATTGGTCTTGTCCAAT
  AAACATGTGGCTCCTGACCACCTACTACAGATATGCCAGCGCATCGGTCCGATGCTGGATAAGGAGGTCC
  CGCCCAGTATCTCAAGAGTCACTTCTTTGCTTGGTGCAGGAAGGCAGTCTTTGCTACGAACAGCAAAAGA
  CTGCAGGCACACAGTGTGGAAGGGCTCTGCCTTTGCTGCTCTTCACAGAGGAAGACCTCCTGAAATGCCC
  GTCAATTATGGCCCCCCACCGAGTCTTGTGGAGATACACCGAGGAAGGCAGCTCACAGGGTGTTCCACTT
  TTAGCACAGCGTTCCCAGGAACTATGTACCAGCATATAAAGATGCACAGGAGGATTCTTGGACACCTGTC
  TGCAGTGTACTGTGTAGCATTTGATAGGACAGGACATAGAATCTTTACAGGTTCAGATGATTGCTTGGTA
  AAGATTTGGTCAACACATAATGGCCGCTTGTTGTCTACGTTAAGAGGCCATTCTGCAGAGATTTCAGACA
  TGGCTGTGAACTACGAGAACACGCTGATTGCTGCAGGAAGCTGTGATAAGATCATTAGGGTGTGGTGCTT
  GAGGACCTGCGCCCCAGTTGCTGTGCTGCAAGGACACACGGGGTCAATCACATCTCTGCAGTTTAGCCCG
  ATGGCCAAAGGACCTCAGCGGTACATGGTTTCTACGGGCGCTGATGGCACTGTTTGCTTCTGGCAGTGGG
  ATTTAGAGTCCTTGAAGTTCAGTCCACGACCCCTGAAGTTCACGGAAAAGCCAAGGCCTGGTGTTCAGAT
  GCTGTGTTCTTCATTTAGCGTTGGTGGTATGTTTTTAGCTACAGGTAGTACTGATCATGTAATCAGAATG
  TATTTTTTGGGATTCGACGCACCTGAAAAAATCGCCGAGCTTGAAAGCCACACTGACAAAGTTGATAGTA
  TCCAGTTTTGTAACAATGGTGACCGGTTCTTAAGTGGTAGCCGAGATGGAACAGCACGGATCTGGAGGTT
  TGAGCAGTTGGAATGGAGAAGCATTTTACTGGACATGTCTGCCAGGATCTCGGGAGACACATCTTCAGAA
  GAAGAGAGGTTTATGAAACCCAAAGTTACAATGATTGCTTGGAACCAGGATGATAGCACCGTGGTCACAG
  CCGTGAATGATCATGTCCTCAAGGTGTGGAGTTCCTACACTGGACAGCTGCTTCATAACTTACTGGGACA
  TGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGAT
  GGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGAC
  AAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCA
  CGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTT
  CATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCAC
  CTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATT
  AGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGAT
  GGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCG
  TCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGAC
  TGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATT
  CAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACG
  CTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGC
  ACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTAT
  GTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGT
  CTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATC
  TTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGAGACATCTTATGATCAGAGTGAAGGTTCTTGTTCT
  TCAGAAGATGACGAGTGGAGAAATGACAGAAGAAGTGGTAGTGACAGCGACAGCTCAAGTGACTCTTCAT
  CTCAGTATTCTGATTGGACAGCTGACACGGGGATCAACTTGCAGCCTCCTCTCAGGATGTCATGTCGTCG
  TCGAGTAACTCGATTCTGCAGTACTTCAGAGGACGAAATGTCCATGGAGAACATATCACCTCCTAAACGA
  AGGCGGAAAAGAAGGAAAGAAAGCAAGCCTAAGAGGGAGAGTTTACGAAGGATGACTCCAGCAGAACTTG
  CAAATATGGAACACCTATATGAGTTTCACCCTCCTGTGTGGATAACGGACACCACACTTCGGAAGTCTCC
  CTTTGTTCCTCAGATGGGTGATGAGGTGATGTACTTCCGTCAGGGTCATGAAGCTTATATTGAAGCTGTC
  AGAAGAAATAACATTTATGAACTAAATCCTCACAAGGAGCCATGGAGGAAAATGGATCTTAGGGATCAAG
  AACTGGTCAAAATTGTTGGACTAAGGTATGAGGTTGGACCACCTACACTTTGTTGCCTCAAACTAGCATT
  TATAGATCCAGCGACTGGCAGACTGACGGATAAATCTTTCTCTATTAGATATCATGACATGCCAGATGTT
  ATTGATTTTCTTGTGTTGCGCCAATTTTATGATGAAGCAAGACAGAGAAACTGGCAGCCTTGTGACAGAT
  TTCGATCTATAATTGATGATGCTTGGTGGTTTGGAACCGTGTTGAGTCAAGAACCGTATCAACCCCAGTA
  TCCTGACAGTCACTTCCAGTGTTACATTGTTAGGTGGGACAACACCGAGACTGAAAAGCTTAGCCCTTGG
  GACATGGAACCGATTCCTGACAATGTTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAG
  ATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTCAAGAGATGAGGAGTG
  TGATCGGATCATCAGTGGCATAGACCAGCTTTTAAACCTCGATATAGCAGCAGCGTTTGCAGGCCCGGTG
  GATCTGTGCACATATCCCAAGTACTGCACAGTAGTGGCTTATCCAACTGATCTCTACACAATTCGAATGA
  GGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGC
  AAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAG
  TTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACA
  GTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAA
  AAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTC
  ATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCAGATTACCGAG
  ATATTATAGATACGCCAATGGATTTTGGAACAGTAAGGGAAACTCTAGAAGCTGGGAATTACGATAGCCC
  TGTGGAGTTTTGCAAAGACATTCGATTGATATTTAGCAATGCAAAGGCATATACACCGAACAAAAGATCA
  AAGATTTATAGTATGACCTTGAGATTGTCTGCTTTGTTTGAAGAAAAAATGAAGAAAATCTCCTTTGATT
  TTAAAATTGGTCAAAAATTCAAGGAGAAACTTCGTAGAAGCCAAAGATTCAAGCAACGACAAAACTGTAA
  CGGTGCTGTCCCTGGTGACAGGCGCAGGAGAAATGTCAAGCAGAAGCAGTTCAAATCTCAGACAAAAGTA
  ATTCCACAGTTGATGTGTCCTCCTTCTCAGTCTACCTCAAGTAAGGTACCTTTGTCTGCAACCCGCAAGA
  CAAGTGCTGGTGTCTCTTCAGGCTTTACTTCTGGGGACTCTTCAGACTCTGCAGGATCTTTAGAAAGAGT
  CAGAAGACAGAGGCCTGAAGTACTCAGGAGTGGTTCTGTGCTGTTTGGAAGTGAAATGGAAGATTTCTTA
  GCTACCTCTTCATCTTCTTCAGCTTCAAACAGTTCAGAGGAAAGCAAAGCGAGTCCTGGAGCTCGAGAAT
  CTTCCTTGCGCAGTGGTGTGTTGAGAGGCAGCAACCTCGGGGTGACCAGAACAAGAGCTGCTCGGAGGAA
  AGCTGGTTCTGTTTCTTTAGAAAATGGGTGTGGTAGAAAAGCCACTCGGAAGAGAGTCTATTTAAGTGAT
  TCTGATAACAATTCAGTGGAAACAGATGAAAATCTGAAAAATAGAAAATGTGGAAGTAGCCGGAAAGTTC
  TGCGGAAGTGTGCTGCTGTTGCTGCCAGCAAGATAAAGCTGATGAGTGATGCAGAGGATTCGAGCTCAGA
  GAGCCCCTGCTCTGGGCGGAAGCTGCCACATCGAAACGCTTCAGCTGTGGCCAGGAAGAAGTTGCTACAC
  AACTCTGATGATCAGAGCCTTAAGTCAGAGACAGAAGAACTGAAAGACCAGAACCAATCACTGTTGATAT
  CCGGTCCCCATTCTGTCCACAACAGCATCAGTGACTCAGAGTCAGACAGTGACTTGCGAGCAACCCGGAA
  GACCTGGAATGCTAACGGCTGCACATCCCACACTGCAGCAACATGCAAAACCAAGTCACGTCCAATAGAG
  TCTTCTGAGGAAGATTCCAGATGTCATGGATCTGATCATGGGCCCAGCAGCACTGGGGACCCTTCAACAT
  CTGGGCAGAAACTCAGGGCAGATAGCATCTCTGAGGAAGCTGACTCTGAACCAGAAAGCAGTGTACTATG
  CAAGAATACACATCTGTGTAAGAAAGCCAAGATCTTGAGTGACTCAGAAGACTGTGAGGAAAAGTGTGGA
  GAAAGGAGAGGTCCTGAAGTGGAAGGAAGCCCAGTTTCTGAAGCCTTGAGGGAAGCCATTCTTGCCCCCC
  AGTGTCTCTCACATCGTGGATCTGAGACTGATGTGGATTCTGACGGCGGTGCAGTGAGAGAGAAATCATA
  CAGCAATGAAAACGGATCTGTATCACTAGAGAATGGACAAAGAAGAAAAGTTTCCCGAAAAAGTTCCAGT
  GACAAGGAAAGTAATCTACAGGTAACCCAGAAGTCACCCAAAGACAGAAGCAGCCCCTCGAGGATTACTC
  AGCGTGCTAGTGTGGCTACTGATAAAATGAAGCTCACAAGTGATGCAGAAGACCTCAGCCTAGAGAGCGT
  GTGTACTCGAAGCAAAAGGAGAAGGAAAAAGCCTGCTCGATTTGCTTGTACCCCAGCAAAGACTGCATTG
  AGTAGTGAGGAGAAACACGCACATTGTGAAGTGCCTGAGGCACAGCCTGCCTGCAGGAACAAGCTCCCTG
  AGCCTGAACACCAAGATAGTGCAGAGAATCCCAGTCAGGCTGCAAGTGCAGACTTGAATTCTGGAGGGGG
  TTCCAGTTTTGAACAAAGAAAAAGCATACAGAGCAGGCAAATGGGCGCTGTCTGCGTAAGGCCACCTCCT
  AAAACCCAAAGCTCCTCTGCTGGGCTTTCTCAGGAAAACGCCAGAAGCCAAACCCTAGATAGTGAGACTA
  GCTTGCCTTCTGAGTCGGTTTTGACCCAGAAAGCTACTGTAGAGAGTAACTTTGAAGAAGAACTGAACTA
  TGGATTAAGAAGGTGGAATGGCAGAAGGCTCAGGACATACGGGAAGGCCCCACTCAGTAGGACAGCACAA
  GTTACTCCCAGTTTACAGGCCTCAGCAGAGGTCGGGGTGAAGAGGAGGAGAATGCATCCTGAGGTAGATG
  GTGAGGACGTGCCCGGACAAATGGGGAGTTCAGGATGCGGACCTGACACCAGTCCCAAGGCATCAGACTT
  GGGGTCAGTAACTGACTCTGATGTTGACTGTACCGATAACACACAAACCCAAAGGAAGAAGAAAAGGAAA
  GGGAAAGCAAGAGTGCTTAGCAAAGGTAAACCCTCCTCATCCAACTCACCTAAAACTGCAAGACATCCAA
  GGCAGAGTAAAGGTCCTAGGTTAAATGTGGATGACAATGACTGGGAGGATTTGGACTATGCAAAAGCTAA
  AGGAGTTGTCCGGCTCTCAAAAATCCAAACGAGAAATCAGGGTAGGAGAACGGTGAGATACCACGATGGG
  GAAGATGATAGAAGCATAGAGAGCGCACTAGAGCTCACTGACCGCACCTTACGCCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001103179
• Insert Size: 6780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001103179.1, NP_001096649.1
• RefSeq Size: 7547 bp
• RefSeq ORF: 6780 bp
• Locus ID: 93871
• UniProt ID: Q921C3
• Cytogenetics: 16 C4
• Gene Summary: May be a transcriptional activator. May be involved in chromatin remodeling. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and CDS compared to variant 1. The resulting isoform (B) is shorter and has a unique C-terminus compared to isoform A.