Dna2 (NM_177372) Mouse Untagged Clone

CAT#: MC223434

Dna2 (untagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dna2
• Synonyms: Dna2l; E130315B21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223434 representing NM_177372
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCTGGACGAGCTGGACCTGCTGCTGCTGGAAGAGGACGGCGGAGCGGAGGCCGTGCCGCGGG
  TGGAGCTACTTCGGAAGAAGGCGGACGCTTTGTTTCCAGAGACAGTTCTGAGCCGAGGAGTGGATAACCG
  GTACCTGGTGCTAGCTGTTGAGACCTCGCAGAATGAAAGAGGAGCCGAGGAGAAGCGCTTGCACGTCACG
  GCGTCCCAGGACCGAGAGCACGAAGTTCTGTGCATCCTGAGGAATGGCTGGAGCTCAGTACCAGTTGAGC
  CAGGGGACATCGTTCACTTAGAGGGAGACTGCACATCTGAGCCGTGGATAATAGACGATGACTTTGGATA
  TTTTATTCTGTACCCGGACATGATGATCTCCGGCACAAGCGTCGCTAGCAGCATTCGGTGTCTGAGAAGA
  GCTGTCCTGAGCGAGACCTTCAGGGGCTCTGATCCAGCCACTCGCCAGATGCTGATCGGTACAATTCTCC
  ACGAAGTCTTTCAGAAAGCGATAAGTGAGAGCTTTGCTCCAGAAAGGCTACAAGAGCTTGCTCTTCAGAC
  CCTCCGAGAAGTGAGGCATTTGAAGGAAATGTATCGCTTGAATCTGAGCCAAGATGAAATACTCTGTGAA
  GTTGAGGAGTATCTCCCTTCTTTCTCTAAGTGGGCGGAGGACTTCATGCGTAAGGGCCCTTCGTCTGAAT
  TCCCTCAAATGCAGCTCTCTCTGCCAAGCGATGGCAGTAACAGAAGTTCACCTTGTAACATTGAAGTAGT
  CAAGTCACTGGATATCGAAGAAAGCATCTGGTCCCCTAGGTTTGGACTGAAGGGCAAAATAGACGTCACA
  GTCGGGGTCAAAATACATCGAGATTGTAAAATGAAATACAAGGTAATGCCACTGGAGCTGAAGACCGGCA
  AAGAGTCCAACTCCATCGAGCACCGGAGTCAGGTGGTCCTGTACACGCTGCTAAGCCAGGAGAGGCGAGA
  GGACCCCGAGGCTGGCTGGCTTCTCTACCTCAAGACCGGGCAGATGTACCCTGTGCCCGCCAACCATCTG
  GACAAAAGAGAACTGCTGAAGCTCAGGAACTGGCTGGCCGCCTCGCTGCTGCACCGTGTGAGCCGAGCAG
  CTCCGGGGGAGGAAGCTCGGCTCTCTGCCTTGCCACAGATAATCGAGGAAGAGAAAACTTGTAAATACTG
  TTCGCAGATAGGCAACTGTGCTCTTTATAGCAGAGCAGTTGAAGAGCAGGGAGATGACGCATCCATCCCA
  GAAGCCATGCTGTCCAAAATCCAGGAAGAAACACGGCACCTGCAGCTGGCGCACTTGAAGTATTTCAGCC
  TGTGGTGTCTCATGTTAACGCTGGAGTCGCAATCTAAAGACAACCGGAAGACTCACCAGAGTATCTGGTT
  AACGCCTGCCTCTGAACTGGAGGAGAGTGGCAACTGTGTTGGAAACCTGGTCAGGACGGAGCCTGTATCA
  AGAGTTTGTGATGGACAGTATTTACATAATTTTCAGCGGAAAAATGGTCCCATGCCAGCCACCAATCTGA
  TGGCAGGTGACAGGATTATTTTAAGTGGAGAAGAGAGAAAACTATTTGCTTTGTCCAAAGGCTACGTGAA
  GAAGATGAACAAGGCAGCCGTTACCTGCTTGCTAGACAGGAACTTGTCGACACTCCCAGCAACAACGGTG
  TTTCGATTAGACCGGGAAGAAAGACATGGCGACATAAGTACTCCACTAGGAAATCTGTCTAAATTGATGG
  AAAGCACAGATCCCAGCAAAAGACTTCGAGAATTAATCATCGATTTCAGGGAACCTCAGTTCATCGCCTA
  CCTCAGCTCCGTCCTTCCACACGATGCGAAGGATACGGTGGCCAACATTCTAAAGGGTTTGAATAAACCC
  CAGAGACAAGCAATGAAGAGAGTCCTTCTTTCGAAAGACTACACGCTCATTGTTGGGATGCCAGGGACAG
  GAAAGACAACCACCATCTGTGCCCTGGTGAGGATCCTCTCTGCCTGTGGGTTCAGCGTTCTGCTGACCAG
  CTACACGCACTCCGCCGTTGACAATATTCTCCTGAAGTTAGCCAAGTTTAAAGTCGGATTTTTGCGCTTG
  GGTCAGTCTCATAAGGTCCATCCAGACATCCAGAAGTTTACAGAGGAGGAGATTTGCAGATCGAGGTCCA
  TTGCGTCTTTAGCCCATCTAGAAGAACTCTATAACAGTCACCCTATTGTTGCAACAACCTGCATGGGAAT
  AAATCACCCAATATTTTCCCGAAAAACCTTTGATTTCTGTATTGTGGATGAAGCATCTCAAATCAGCCAG
  CCCGTCTGCCTGGGCCCACTGTTCTTTTCGCGGAGGTTTGTGCTGGTGGGGGACCATCAGCAGCTGCCTC
  CCCTGGTTGTAAACCGAGAGGCAAGAGCTCTGGGCATGAGCGAGAGCTTGTTCAAGAGGCTGGAGCGGAA
  CGAGAGCGCTGTGGTGCAGCTGACCGTGCAATACCGAATGAACAGGAAGATTATGTCCCTGAGCAACAAG
  CTCACATACGCAGGGAAACTGGAGTGTGGGTCAGACCGAGTGGCCAACGCAGTGCTAGCCCTGCCCAACC
  TCAAGGACGCAAGGCTGAGCCTCCAGCTTTATGCCGACTACTCTGACAGCCCCTGGCTGGCTGGAGTGTT
  AGAGCCAGACAATCCCGTGTGTTTCCTTAACACAGACAAGGTCCCAGCTCCAGAGCAAGTTGAAAATGGT
  GGTGTTAGCAATGTCACAGAAGCCAGACTCATTGTCTTCCTAACCTCAACTTTTATAAAGGCAGGCTGCA
  GCCCCTCAGACATTGGCGTCATCGCCCCGTACCGACAGCAGCTGCGGATCATCAGCGACTTACTGGCCCG
  GTCTTCTGTTGGGATGGTTGAGGTTAACACAGTAGACAAATACCAAGGAAGGGACAAGAGTCTCATCCTG
  GTGTCCTTTGTTAGGAGTAATGAGGATGGAACTCTCGGCGAGCTCCTGAAAGATTGGCGGAGACTCAACG
  TGGCACTCACCAGAGCCAAGCATAAACTGATCCTGCTGGGCAGCGTGTCCTCGCTGAAGCGCTTCCCGCC
  TCTGGGGACGCTCTTTGACCATCTGAATGCAGAGCAGTTAATCCTGGACCTTCCTTCCAGAGAGCATGAG
  AGCCTCAGCCACATCCTGGGCGATTGTCAGCGAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177372
• Insert Size: 3189 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177372.3, NP_796346.2
• RefSeq Size: 4122 bp
• RefSeq ORF: 3189 bp
• Locus ID: 327762
• UniProt ID: Q6ZQJ5
• Cytogenetics: 10 B4
• Gene Summary: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity).[UniProtKB/Swiss-Prot Function]