Smarca5 (NM_053124) Mouse Untagged Clone

CAT#: MC223354

Smarca5 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (Smarca5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smarca5
• Synonyms: 4933427E24Rik; D030040M08Rik; D330027N15Rik; MommeD4; Snf2h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223354 representing NM_053124
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCGCGGTGGAGCCTCCGCCGCCCCCGCCTCCCGAGAGCGCGCCTTCCAAGCCCTCGGCGGCGG
  GCGCCGGCGGGAGCAGCAGCGGCAACAAAGGCGGCCCCGAGGGCGGCGCGGCGCCCGCGGCTCCGTGTGC
  TGCGGGCTCGGGCCCCGCGGACACCGAGATGGAGGAAGTATTTGATCATGGATCACCTGGAAAGCAAAAA
  GAAATCCAAGAACCAGATCCTACATATGAAGAAAAAATGCAAACTGACCGAGCAAATAGATTTGAGTATT
  TATTAAAGCAGACAGAGCTGTTCGCACATTTCATTCAGCCTGCTGCTCAGAAGACTCCAACCTCACCCTT
  GAAGATGAAACCAGGGCGCCCACGGGTAAAAAAGGATGAAAAACAGAATTTGCTTTCAGTTGGAGATTAC
  CGACACCGTAGAACGGAGCAAGAGGAGGATGAAGAGCTATTAACAGAAAGTTCCAAAGCAACTAATGTTT
  GTACTCGATTTGAAGATTCTCCATCATATGTAAAATGGGGTAAACTGAGAGATTATCAGGTGCGAGGATT
  GAATTGGCTCATCTCTCTGTATGAGAATGGCATCAATGGGATCCTTGCAGATGAAATGGGTTTGGGAAAG
  ACACTTCAAACAATTTCTCTTCTTGGATACATGAAACACTATAGAAATATTCCTGGTCCTCATATGGTTT
  TGGTTCCCAAGTCTACATTGCACAACTGGATGAGTGAATTCAAGAAATGGGTACCAACACTTAGGTCTGT
  TTGCTTGATAGGAGATAAAGAGCAAAGAGCTGCATTTGTCAGGGATGTTTTATTACCAGGAGAGTGGGAT
  GTGTGTGTCACATCTTATGAAATGCTTATTAAAGAGAAGTCTGTGTTCAAAAAATTCAACTGGAGATACT
  TAGTTATAGATGAAGCTCACAGGATAAAAAATGAAAAATCTAAGTTGTCAGAAATAGTGAGAGAATTCAA
  GACTACAAATCGGCTGTTGCTAACAGGGACGCCTCTTCAGAACAACCTGCATGAGCTCTGGTCACTTCTA
  AACTTTTTGTTACCAGATGTGTTTAATTCAGCTGATGACTTTGATTCCTGGTTTGATACAAATAATTGCC
  TTGGGGATCAAAAGCTAGTTGAGAGGCTTCATATGGTTTTGCGTCCATTCCTCCTTCGTCGAATTAAAGC
  TGATGTTGAAAAGAGTTTGCCTCCAAAAAAAGAAGTAAAAATCTATGTGGGTCTTAGCAAAATGCAAAGA
  GAATGGTATACTCGGATATTAATGAAAGATATAGACATATTAAACTCAGCAGGGAAGATGGACAAAATGA
  GGCTATTGAACATTTTGATGCAGTTGAGGAAATGCTGCAATCATCCTTATCTCTTCGATGGAGCTGAACC
  TGGTCCACCGTACACAACAGATATGCATCTAGTTACCAACAGTGGCAAGATGGTGGTGTTAGACAAGCTA
  CTCCCTAAACTGAAAGAACAAGGTTCAAGAGTACTAATCTTTAGTCAGATGACAAGAGTATTAGACATTT
  TGGAAGATTATTGCATGTGGAGAAATTATGAGTACTGCAGGTTGGATGGACAGACACCCCATGATGAGAG
  ACAAGACTCCATCAATGCATACAATGAACCAAATAGCACAAAGTTTGTATTTATGCTAAGCACACGTGCT
  GGTGGTCTGGGCATCAATCTTGCAACTGCTGATGTAGTCATTTTATATGACTCAGATTGGAATCCGCAAG
  TTGATCTTCAGGCTATGGACCGTGCCCATAGAATTGGACAAACGAAGACTGTCCGAGTGTTTCGCTTTAT
  AACTGATAACACTGTGGAAGAAAGAATAGTGGAACGTGCTGAGATGAAACTTAGACTGGATTCGATAGTA
  ATTCAACAAGGGAGGCTTGTAGATCAGAACCTGAACAAAATTGGGAAAGATGAAATGCTTCAAATGATTC
  GACATGGTGCTACACATGTGTTTGCTTCAAAAGAAAGTGAAATCACTGATGAGGATATTGATGGTATTTT
  AGAAAGAGGTGCAAAGAAGACTGCAGAAATGAACGAAAAGCTCTCCAAGATGGGTGAAAGTTCACTTAGA
  AACTTTACGATGGACACAGAGTCCAGTGTTTATAACTTTGAAGGAGAAGACTATAGAGAAAAACAAAAGA
  TTGCATTCACAGAGTGGATTGAACCACCAAAAAGAGAAAGAAAAGCCAACTATGCTGTTGATGCATATTT
  CAGGGAAGCTCTCCGTGTTAGTGAGCCTAAAGCACCCAAGGCACCTCGACCTCCAAAACAACCAAATGTT
  CAGGATTTCCAGTTCTTTCCTCCGCGTTTATTTGAATTACTGGAAAAAGAAATTCTGTATTACAGGAAAA
  CTATTGGGTATAAGGTACCTCGGAGTCCTGATCTACCTAATGCAGCACAGGCACAGAAAGAGGAACAGCT
  AAAAATTGATGAAGCAGAGCCCCTCAATGACGAGGAGTTAGAGGAAAAAGAGAAGCTTCTGACACAGGGG
  TTTACAAATTGGAATAAGAGAGATTTTAACCAGTTTATCAAGGCTAATGAGAAGTGGGGTCGTGATGATA
  TTGAAAATATAGCCAGAGAAGTAGAAGGAAAAACTCCAGAAGAAGTCATTGAATACTCAGCTGTGTTCTG
  GGAAAGATGCAATGAGCTTCAGGACATAGAGAAGATTATGGCTCAGATTGAAAGGGGAGAGGCAAGAATT
  CAAAGAAGAATAAGTATCAAGAAAGCACTAGATACAAAGATTGGACGGTACAAAGCACCCTTTCATCAGC
  TAAGAATATCATATGGTACTAACAAAGGAAAAAACTATACTGAAGAAGAGGATCGTTTTCTCATTTGTAT
  GCTGCACAAGCTTGGCTTCGACAAGGAAAATGTGTATGATGAATTACGACAATGCATCCGAAACTCCCCT
  CAGTTTAGATTTGATTGGTTTCTCAAGTCCAGAACTGCGATGGAGCTCCAGAGGAGGTGTAATACCTTAA
  TTACTTTGATTGAAAGAGAAAATATGGAACTAGAAGAAAAGGAGAAGGCAGAGAAAAAGAAAAGAGGACC
  AAAACCTTCCACACAGAAACGTAAGATGGACGGTGCCCCTGATGGCCGTGGAAGAAAAAAGAAGCTGAAA
  CTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053124
• Insert Size: 3156 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053124.2, NP_444354.2
• RefSeq Size: 4658 bp
• RefSeq ORF: 3156 bp
• Locus ID: 93762
• UniProt ID: Q91ZW3
• Cytogenetics: 8 C2
• Gene Summary: Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.[UniProtKB/Swiss-Prot Function]