Smarca5 (NM_053124) Mouse Untagged Clone
CAT#: MC223354
Smarca5 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (Smarca5), (10ug)
"NM_053124" in other vectors (3)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Smarca5 |
Synonyms | 4933427E24Rik; D030040M08Rik; D330027N15Rik; MommeD4; Snf2h |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC223354 representing NM_053124
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCGCGGTGGAGCCTCCGCCGCCCCCGCCTCCCGAGAGCGCGCCTTCCAAGCCCTCGGCGGCGG GCGCCGGCGGGAGCAGCAGCGGCAACAAAGGCGGCCCCGAGGGCGGCGCGGCGCCCGCGGCTCCGTGTGC TGCGGGCTCGGGCCCCGCGGACACCGAGATGGAGGAAGTATTTGATCATGGATCACCTGGAAAGCAAAAA GAAATCCAAGAACCAGATCCTACATATGAAGAAAAAATGCAAACTGACCGAGCAAATAGATTTGAGTATT TATTAAAGCAGACAGAGCTGTTCGCACATTTCATTCAGCCTGCTGCTCAGAAGACTCCAACCTCACCCTT GAAGATGAAACCAGGGCGCCCACGGGTAAAAAAGGATGAAAAACAGAATTTGCTTTCAGTTGGAGATTAC CGACACCGTAGAACGGAGCAAGAGGAGGATGAAGAGCTATTAACAGAAAGTTCCAAAGCAACTAATGTTT GTACTCGATTTGAAGATTCTCCATCATATGTAAAATGGGGTAAACTGAGAGATTATCAGGTGCGAGGATT GAATTGGCTCATCTCTCTGTATGAGAATGGCATCAATGGGATCCTTGCAGATGAAATGGGTTTGGGAAAG ACACTTCAAACAATTTCTCTTCTTGGATACATGAAACACTATAGAAATATTCCTGGTCCTCATATGGTTT TGGTTCCCAAGTCTACATTGCACAACTGGATGAGTGAATTCAAGAAATGGGTACCAACACTTAGGTCTGT TTGCTTGATAGGAGATAAAGAGCAAAGAGCTGCATTTGTCAGGGATGTTTTATTACCAGGAGAGTGGGAT GTGTGTGTCACATCTTATGAAATGCTTATTAAAGAGAAGTCTGTGTTCAAAAAATTCAACTGGAGATACT TAGTTATAGATGAAGCTCACAGGATAAAAAATGAAAAATCTAAGTTGTCAGAAATAGTGAGAGAATTCAA GACTACAAATCGGCTGTTGCTAACAGGGACGCCTCTTCAGAACAACCTGCATGAGCTCTGGTCACTTCTA AACTTTTTGTTACCAGATGTGTTTAATTCAGCTGATGACTTTGATTCCTGGTTTGATACAAATAATTGCC TTGGGGATCAAAAGCTAGTTGAGAGGCTTCATATGGTTTTGCGTCCATTCCTCCTTCGTCGAATTAAAGC TGATGTTGAAAAGAGTTTGCCTCCAAAAAAAGAAGTAAAAATCTATGTGGGTCTTAGCAAAATGCAAAGA GAATGGTATACTCGGATATTAATGAAAGATATAGACATATTAAACTCAGCAGGGAAGATGGACAAAATGA GGCTATTGAACATTTTGATGCAGTTGAGGAAATGCTGCAATCATCCTTATCTCTTCGATGGAGCTGAACC TGGTCCACCGTACACAACAGATATGCATCTAGTTACCAACAGTGGCAAGATGGTGGTGTTAGACAAGCTA CTCCCTAAACTGAAAGAACAAGGTTCAAGAGTACTAATCTTTAGTCAGATGACAAGAGTATTAGACATTT TGGAAGATTATTGCATGTGGAGAAATTATGAGTACTGCAGGTTGGATGGACAGACACCCCATGATGAGAG ACAAGACTCCATCAATGCATACAATGAACCAAATAGCACAAAGTTTGTATTTATGCTAAGCACACGTGCT GGTGGTCTGGGCATCAATCTTGCAACTGCTGATGTAGTCATTTTATATGACTCAGATTGGAATCCGCAAG TTGATCTTCAGGCTATGGACCGTGCCCATAGAATTGGACAAACGAAGACTGTCCGAGTGTTTCGCTTTAT AACTGATAACACTGTGGAAGAAAGAATAGTGGAACGTGCTGAGATGAAACTTAGACTGGATTCGATAGTA ATTCAACAAGGGAGGCTTGTAGATCAGAACCTGAACAAAATTGGGAAAGATGAAATGCTTCAAATGATTC GACATGGTGCTACACATGTGTTTGCTTCAAAAGAAAGTGAAATCACTGATGAGGATATTGATGGTATTTT AGAAAGAGGTGCAAAGAAGACTGCAGAAATGAACGAAAAGCTCTCCAAGATGGGTGAAAGTTCACTTAGA AACTTTACGATGGACACAGAGTCCAGTGTTTATAACTTTGAAGGAGAAGACTATAGAGAAAAACAAAAGA TTGCATTCACAGAGTGGATTGAACCACCAAAAAGAGAAAGAAAAGCCAACTATGCTGTTGATGCATATTT CAGGGAAGCTCTCCGTGTTAGTGAGCCTAAAGCACCCAAGGCACCTCGACCTCCAAAACAACCAAATGTT CAGGATTTCCAGTTCTTTCCTCCGCGTTTATTTGAATTACTGGAAAAAGAAATTCTGTATTACAGGAAAA CTATTGGGTATAAGGTACCTCGGAGTCCTGATCTACCTAATGCAGCACAGGCACAGAAAGAGGAACAGCT AAAAATTGATGAAGCAGAGCCCCTCAATGACGAGGAGTTAGAGGAAAAAGAGAAGCTTCTGACACAGGGG TTTACAAATTGGAATAAGAGAGATTTTAACCAGTTTATCAAGGCTAATGAGAAGTGGGGTCGTGATGATA TTGAAAATATAGCCAGAGAAGTAGAAGGAAAAACTCCAGAAGAAGTCATTGAATACTCAGCTGTGTTCTG GGAAAGATGCAATGAGCTTCAGGACATAGAGAAGATTATGGCTCAGATTGAAAGGGGAGAGGCAAGAATT CAAAGAAGAATAAGTATCAAGAAAGCACTAGATACAAAGATTGGACGGTACAAAGCACCCTTTCATCAGC TAAGAATATCATATGGTACTAACAAAGGAAAAAACTATACTGAAGAAGAGGATCGTTTTCTCATTTGTAT GCTGCACAAGCTTGGCTTCGACAAGGAAAATGTGTATGATGAATTACGACAATGCATCCGAAACTCCCCT CAGTTTAGATTTGATTGGTTTCTCAAGTCCAGAACTGCGATGGAGCTCCAGAGGAGGTGTAATACCTTAA TTACTTTGATTGAAAGAGAAAATATGGAACTAGAAGAAAAGGAGAAGGCAGAGAAAAAGAAAAGAGGACC AAAACCTTCCACACAGAAACGTAAGATGGACGGTGCCCCTGATGGCCGTGGAAGAAAAAAGAAGCTGAAA CTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_053124 |
Insert Size | 3156 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053124.2, NP_444354.2 |
RefSeq Size | 4658 bp |
RefSeq ORF | 3156 bp |
Locus ID | 93762 |
UniProt ID | Q91ZW3 |
Cytogenetics | 8 C2 |
Gene Summary | Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR211537 | Smarca5 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (Smarca5) |
USD 915.00 |
|
MR211537L3 | Lenti ORF clone of Smarca5 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (Smarca5) |
USD 1,215.00 |
|
MR211537L4 | Lenti ORF clone of Smarca5 (mGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (Smarca5) |
USD 1,215.00 |
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