Lig4 (NM_176953) Mouse Untagged Clone

CAT#: MC222564

Lig4 (untagged) - Mouse ligase IV, DNA, ATP-dependent (Lig4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lig4
• Synonyms: 5830471N16Rik; tiny
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222564 representing NM_176953
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCTCACAAACTTCACAAACTGTTGCAGCTCATGTCCCCTTTGCAGACTTATGTTCCACACTAG
  AACGGATACAGAAAGGTAAAGACCGTGCAGAAAAAATCAGGCACTTCAAGGAGTTTTTGGATTCGTGGAG
  AAAATTTCATGATGCCCTTCATAAGAACAGAAAGGACGTTACAGACTCTTTTTACCCTGCAATGAGACTC
  ATTCTCCCCCAGTTAGAAAGAGAGAGGATGGCTTATGGAATCAAAGAAACCATGCTCGCTAAGCTCTACA
  TCGAATTGCTGAACTTACCACGAGAAGGCAAGGATGCCCAGAAGCTCCTCAATTACCGAACCCCCAGTGG
  AGCTCGCACGGATGCTGGGGACTTTGCCATGATTGCATACTTTGTTTTGAAGCCAAGGTGCTTACAGAAA
  GGAAGCTTAACCATACAGCAGGTAAATGAACTCTTAGACTTAGTTGCCAGCAATAACTCTGGCAAAAAAA
  AAGACCTAGTGAAAAAGAGCCTTCTTCAGTTAATAACCCAGAGTTCAGCACTGGAGCAAAAATGGCTGAT
  TCGCATGATTATCAAAGACTTAAAGCTTGGCATCAGTCAGCAAACAATATTTTCCATTTTCCACAATGAT
  GCAGTTGAGTTGCACAACGTCACCACAGATCTGGAAAAGGTCTGCAGGCAGCTGCATGACCCCTCTGTAG
  GGCTTAGTGACATCTCTATCACTCTGTTTTCTGCCTTTAAGCCAATGCTAGCTGCTGTAGCAGACGTGGA
  GCGTGTGGAGAAGGACATGAAGCAGCAGAGTTTCTACATCGAAACTAAGCTTGATGGTGAGCGCATGCAG
  ATGCACAAAGATGGCGCGCTGTACCGGTACTTCTCCAGAAACGGTTACAACTATACCGACCAGTTTGGTG
  AATCTCCACAGGAAGGCTCTCTCACCCCATTTATTCACAATGCGTTCGGGACAGATGTGCAAGCGTGCAT
  CCTTGACGGTGAGATGATGGCCTACAACCCAACAACACAGACTTTCATGCAGAAGGGGGTCAAGTTTGAT
  ATCAAAAGGATGGTGGAAGATTCTGGCCTACAGACTTGTTACTCTGTTTTTGACGTACTGATGGTTAATA
  AGAAGAAGCTAGGGCGCGAGACTCTTAGGAAGAGGTATGAGATCCTTAGTAGCACTTTCACACCCATACA
  AGGCCGCATAGAAATAGTGCAGAAAACTCAAGCTCATACAAAGAAGGAAGTAGTGGATGCATTAAATGAT
  GCCATAGACAAGAGAGAAGAGGGGATCATGGTTAAACACCCTCTGTCCATTTACAAGCCAGACAAAAGAG
  GTGAAGGGTGGCTAAAGATTAAACCAGAGTACGTCAGTGGACTAATGGATGAATTAGACGTCCTAATTGT
  GGGGGGCTACTGGGGTAAAGGTTCACGAGGTGGCATGATGTCTCACTTTTTGTGTGCAGTGGCAGAGACA
  CCACCTCCTGGTGACAGGCCATCTGTATTCCACACTCTGTGCCGTGTTGGGTCTGGTTACACCATGAAAG
  AACTCTATGACCTGGGCTTGAAATTGGCAAAATACTGGAAGCCTTTTCATAAGAAATCTCCACCAAGTAG
  CATTCTGTGTGGTACAGAGAAGCCGGAAGTGTACATAGAGCCTCAGAACTCTGTCATTGTTCAGATCAAG
  GCAGCTGAGATCGTCCCCAGTGACATGTACAAGACTGGCTCCACCCTGCGCTTCCCACGCATCGAAAAGA
  TCAGAGATGACAAAGAGTGGCACGAATGTATGACACTGGGTGACTTGGAGCAGCTGAGGGGGAAAGCATC
  TGGGAAGCTTGCCACGAAACACCTTCATGTAGGTGATGATGATGAACCTAGAGAAAAAAGGCGGAAGCCC
  ATCTCCAAGACGAAGAAAGCCATTAGAATCATTGAACACTTAAAAGCACCCAACCTTTCTAACGTAAACA
  AAGTTTCCAATGTATTTGAAGATGTTGAGTTTTGTGTTATGAGTGGATTAGATGGTTATCCAAAGGCTGA
  CCTAGAGAACAGAATTGCAGAATTCGGTGGTTATATAGTACAGAATCCAGGCCCGGATACATACTGTGTT
  ATTGCAGGTTCTGAGAACGTTAGAGTGAAAAACATTATTTCTTCAGATAAAAATGATGTTGTCAAGCCCG
  AGTGGCTTTTAGAGTGTTTTAAGACAAAAACATGCGTGCCGTGGCAACCTCGCTTTATGATTCACATGTG
  CCCGTCGACAAAGCAGCATTTTGCCCGTGAGTATGACTGCTATGGTGATAGCTATTTTGTTGACACAGAT
  TTGGATCAATTGAAAGAAGTGTTTCTAGGAATTAAACCCAGTGAGCAGCAGACTCCTGAAGAAATGGCCC
  CTGTGATTGCTGACTTAGAATGTCGTTATTCCTGGGACCACTCTCCTCTCAGTATGTTTCGACATTACAC
  CATTTATTTGGACTTGTATGCTGTTATTAATGACTTGAGTTCCAGAATTGAAGCCACGAGATTAGGTATT
  ACAGCCCTTGAGCTGCGGTTTCATGGAGCAAAGGTGGTTTCCTGCTTATCTGAAGGGGTATCTCATGTTA
  TCATTGGGGAGGATCAGAGACGAGTTACTGACTTTAAAATATTCAGAAGAATGCTTAAGAAAAAGTTTAA
  AATCCTGCAAGAAAGTTGGGTGTCCGATTCAGTAGACAAGGGCGAACTGCAGGAGGAAAACCAGTATTTG
  CTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_176953
• Insert Size: 2736 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176953.3, NP_795927.2
• RefSeq Size: 4081 bp
• RefSeq ORF: 2736 bp
• Locus ID: 319583
• UniProt ID: Q8BTF7
• Cytogenetics: 8 A1.1
• Gene Summary: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.[UniProtKB/Swiss-Prot Function]