Rpa1 (NM_026653) Mouse Untagged Clone

CAT#: MC219833

Rpa1 (untagged) - Mouse replication protein A1 (Rpa1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rpa1
• Synonyms: 70kDa; 5031405K23Rik; AA589576; AW557552; RF-A; RP-A; Rpa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219833 representing NM_026653
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGACACCTGAGCGAGGGGGCCATCGAGGTCATGATACAGCAGGAAAATACATCCATAAAGCCCA
  TCCTGCAGGTCATTAACATCCGTCCCATTTCTACAGGGAATAGGTCACCCCGGTACCGACTGCTCATGAG
  TGATGGGTTGAACACGCTTTCCTCGTTCATGCTGGCCACGCAGCTGAACACCCTGGTCGAGGGAGGGCAG
  CTGGCCAGCAACTGTGTCTGCCAGGTCCACAAGTTCATCGTCAACACCCTGAAAGACGGCAGGAAAGTAG
  TTGTTTTGATGGACTTGGAAGTTATGAAATCTGCTGAAGACGTTGGATTAAAGATTGGGAATCCAGTGCC
  CTATAATGAAGGATATGGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAGCAGTCCCTTCCCCA
  GCCTCTGCAGCGACACCACCAGCCAGCAAGCCCCAGCCACAGAATGGGAGTCTGGGAATGGGTTCCACTG
  CAGCTAAGGCTTATGGTGCCTCAAAGCCATTTGGAAAACCTGCCGGAACTGGCCTGCTGCAGCCTTCAGG
  TGGGACACAGTCCAAAGTGGTGCCCATCGCCAGCCTCACCCCGTACCAGTCCAAGTGGACTATTTGTGCC
  CGTGTCACCAACAAAAGTCAGATCCGCACCTGGAGTAATTCCCGGGGGGAAGGAAAGCTTTTCTCTCTAG
  AACTTGTGGATGAAAGCGGTGAAATCAGAGCTACTGCTTTCAATGAGCAAGTGGACAAGTTCTTTCCCCT
  TATTGAAGTGAACAAGGTGTATTACTTCTCAAAGGGCGCCCTGAAGATCGCTAACAAACAGTTCTCCGCT
  GTTAAAAATGACTATGAGATGACCTTCAATAATGAGACTTCTGTCCTTCCCTGTGAAGATGGCCATCACT
  TACCCACAGTTCAGTTTGATTTCACAGGGATCGGTGACCTAGAGAGCAAGGCTAAAGACGCACTAGTAGA
  CATCATTGGAATCTGCAAGAGCTATGAAGATTCGATTAAAATCACAGTGAAGTCGAACAACAGAGAAGTT
  GCTAAGAGAAATATCTATTTGATGGACATGTCAGGGAAGGTGGTGACCACAACTCTGTGGGGAGAAGATG
  CTGACAAGTTTGACGGCTCTCGGCAGCCCGTGATGGCCATCAAAGGAGCCCGAGTCTCTGACTTCGGTGG
  ACGGAGCCTCTCGGTCCTCTCATCCAGCACTGTCATTGTGAATCCTGACATCCCAGAGGCCTATAAGCTT
  CGTGGCTGGTTTGACTCAGAAGGACAAGCCTTAGATGGTGTTTCCATCTCTGACCACAGGAGTGGAGGGG
  CCGGAGGGGGCAACACCAACTGGAAAACTTTGCATGAGGCTAAATCTGAGAACCTGGGCCAGGGAGACAA
  GGCGGACTATTTCAGCACTGTGGCAGCGGTGGTGTTTCTCCGCAAAGAGAACTGTATGTACCAGGCCTGC
  CCAACCCAGGACTGCAATAAGAAAGTGATTGACCAGCAGAATGGGTTGTACCGCTGTGAGAAGTGTGACC
  GGGAATTTCCCAATTTCAAATACCGCATGATCTTATCGGCAAATATTGCAGATTTTCAAGAGAACCAGTG
  GGTGACGTGTTTCCAGGAGTCTGCAGAGGCCATCCTTGGACAGAACACTATGTACCTTGGGGAACTCAAA
  GAGAAGAACGAGCAGGCGTTTGAAGAGGTTTTCCAGAATGCCAACTTCCGGTCATTTACGTTCAGAATCA
  GGGTCAAGCTGGAGACCTACAATGATGAATCTCGAATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGA
  CTTCAGAGACTATGGCAGACGGCTGATCGCGAACATCAGGAAGAACATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026653
• Insert Size: 1872 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026653.2, NP_080929.1
• RefSeq Size: 3058 bp
• RefSeq ORF: 1872 bp
• Locus ID: 68275
• UniProt ID: Q8VEE4
• Cytogenetics: 11 45.79 cM
• Gene Summary: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an alternate segment, compared to isoform 1.