Hnf1b (NM_009330) Mouse Untagged Clone

CAT#: MC218963

Hnf1b (untagged) - Mouse HNF1 homeobox B (Hnf1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hnf1b
• Synonyms: HNF-1-beta; HNF-1B; HNF-1Beta; Hnf1beta; LFB3; Tcf-2; Tcf2; vHNF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218963 representing NM_009330
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCCAAGCTCACGTCGCTCCAGCAAGAACTCCTGAGTGCCCTGCTGAGCTCCGGAGTCACCAAGG
  AAGTGCTGATCCAGGCCTTGGAGGAGTTACTGCCGTCCCCGAATTTCGGGGTGAAGCTGGAGACACTGCC
  CCTGTCCCCCGGGAGCGGGGCGGATCTCGACACCAAGCCGGTTTTCCATACTCTCACCAATGGCCACGCC
  AAGGGCCGCTTGTCTGGGGACGAGGGCTCAGAGGACGGCGACGACTATGACACTCCTCCCATCCTCAAAG
  AGCTCCAGGCGCTCAACACCGAGGAGGCCGCGGAGCAGCGGGCCGAGGTGGACCGGATGCTCAGCGAGGA
  CCCGTGGAGGGCTGCCAAAATGATCAAGGGATACATGCAACAGCACAATATCCCCCAGAGGGAGGTGGTC
  GATGTCACAGGCCTGAACCAATCCCACCTCTCTCAACACCTCAACAAGGGCACCCCCATGAAGACCCAGA
  AGAGAGCTGCCCTGTACACTTGGTACGTCAGAAAGCAACGGGAGATCCTCCGACAGTTCAACCAGACAGT
  CCAGAGCTCTGGAAACATGACAGACAAAAGCAGTCAGGATCAGCTGCTGTTTCTCTTTCCAGAGTTCAGT
  CAACAGAACCAGGGGCCTGGGCAGTCGGAGGACACCTGCTCCGAGCCCACCAACAAGAAGATGCGCCGCA
  ACCGGTTTAAATGGGGGCCCGCATCCCAGCAAATTTTGTACCAGGCCTACGACCGGCAAAAGAATCCCAG
  CAAGGAAGAGAGGGAGGCCTTAGTGGAGGAGTGTAACAGGGCAGAATGTTTGCAACGAGGGGTCTCCCCT
  TCCAAAGCCCACGGCCTAGGCTCCAACTTGGTCACGGAGGTCCGTGTCTACAACTGGTTCGCAAACCGCC
  GGAAGGAAGAGGCGTTCAGGCAGAAGCTGGCCATGGATGCCTATAGCTCCAACCAGACGCACAACCTGAA
  CCCCCTGCTCACCCATGGCTCCCCTCACCATCAGCCAAGCTCCTCTCCACCCAACAAGATGTCAGGAGTG
  CGCTACAACCAGCCGGGAAACAATGAGGTCACTTCCTCTTCGACAATCAGTCACCATGGTAACAGTGCCA
  TGGTGACCAGCCAGTCGGTTTTACAGCAAGTCTCCCCAGCCAGCCTGGACCCAGGCCACAGTCTCCTCTC
  ACCTGACAGTAAAATGCAGATCACAGTGTCGGGAGGAGGACTGCCTCCCGTCAGCACCTTGACGAATATC
  CACAGCCTCTCCCACCACAATCCCCAGCAATCTCAGAACCTCATCATGACCCCCCTGTCTGGAGTCATGG
  CCATTGCACAGAGCCTCAACACCTCCCAAGCCCAGGGTGTCCCGGTCATTAACAGTGTGGCTAGCAGCCT
  GGCAGCCCTACAGCCCGTCCAGTTCTCTCAACAGCTGCACAGCCCTCACCAGCAGCCCCTCATGCAGCAG
  AGCCCAGGCAGTCACATGGCCCAGCAGCCCTTCATGGCCGCGGTGACTCAGCTACAGAACTCCCACATGT
  ATGCACATAAGCAGGAACCCCCCCAGTATTCCCACACCTCCCGGTTCCCATCTGCAATGGTGGTCACAGA
  TACCAGTAGCATCAACACTCTCACCAGCATGTCTTCCAGTAAACAGTGTCCACTGCAAGCCTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009330
• Insert Size: 1677 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009330.3, NP_033356.2
• RefSeq Size: 2843 bp
• RefSeq ORF: 1677 bp
• Locus ID: 21410
• UniProt ID: P27889
• Cytogenetics: 11 51.23 cM
• Gene Summary: Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).