Ppm1b (NM_001159496) Mouse Untagged Clone

CAT#: MC216476

Ppm1b (untagged) - Mouse protein phosphatase 1B, magnesium dependent, beta isoform (Ppm1b), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppm1b
• Synonyms: PP2CB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216476 representing NM_001159496
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCATTTTTGGATAAACCCAAAACTGAAAAGCACAATGCTCACGGTGCTGGGAATGGTCTGCGTT
  ATGGCCTGAGCAGTATGCAAGGATGGAGAGTAGAAATGGAAGATGCACACACAGCTGTTGTGGGTATTCC
  TCACGGCTTGGACAACTGGTCGTTTTTTGCAGTTTATGACGGTCATGCTGGATCCCGAGTGGCAAATTAC
  TGTTCAACACATCTATTAGAACACATCACCACCAATGAAGACTTCAGGGCAGCTGACAAATCAGGCTCTG
  CTCTCGAGCCTTCAGTAGAAAGTGTGAAGACTGGTATCAGGACTGGCTTTTTGAAAATTGATGAATATAT
  GCGTAACTTCTCAGACCTGAGGAACGGGATGGACAGGAGCGGCTCGACTGCAGTGGGCGTGATGGTCTCC
  CCTACACACATGTACTTCATCAACTGTGGTGACTCTCGAGCTGTTCTGTGTAGGAACGGACAGGTCTGCT
  TTTCTACCCAGGATCACAAACCTTGTAATCCAGTGGAGAAGGAGCGCATCCAAAACGCAGGAGGCAGTGT
  GATGATCCAGCGTGTGAACGGCTCACTAGCAGTGTCTCGGGCTCTGGGGGACTATGACTACAAGTGTGTG
  GATGGCAAGGGCCCTACAGAGCAGCTTGTTTCTCCAGAGCCTGAGGTTTATGAGATTGTGAGAGCAGAAG
  AGGATGAGTTTGTCGTCTTGGCCTGTGATGGGATCTGGGATGTGATGAGCAATGAGGAGCTCTGTGAGTT
  TGTTAAGTCTAGGCTTGAGGTGTCGGACGACCTGGAGAATGTGTGCAATTGGGTAGTGGACACTTGTTTA
  CATAAGGGAAGTCGAGATAACATGAGTGTTGTATTAGTTTGCTTTTCAAATGCCCCCAAGGTCTCAGAGG
  AAGCCGTGAAGAGAGATTCAGAGTTGGATAAGCACTTGGAATCACGGGTTGAAGAAATCATGCAGAAGTC
  TGGGGAGGAAGGAATGCCTGATCTTGCCCATGTGATGCGCATTTTGTCTGCAGAAAATATCCCGAATTTA
  CCTCCCGGGGGAGGTCTCGCTGGCAAGCGCCATGTTATTGAAGCTGTTTATAGTAGACTTAATCCACACA
  AAGACAATGATGGGGCCTCCGATGAAGCAGAGGAAGGTGGATCACAGGGGAAATTGGTGGAAGCTCTCAG
  GCAAATGAGAGTTAACCATAGGGGAAACTACCGACAGCTGCTGGAGGAGATGCTGACTAGTTACAGGCTA
  GCTAAAGTAGAGGGAGAAGAGAGCCCGGCTGACCCAGCGGCTGCAGCTGCTTCTTCGAACAGTGATGGTG
  GAAACCCAGTGGCAATGCAGGAGAGAGACACTGAGGGTGGTCCTGCTGGCCTAGACAGCCGTAATGAAGA
  TGCAGGGACAAAGAGGAGTGCTGAAAATATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159496
• Insert Size: 1434 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159496.1, NP_001152968.1
• RefSeq Size: 2753 bp
• RefSeq ORF: 1434 bp
• Locus ID: 19043
• Cytogenetics: 17 55.13 cM
• Gene Summary: Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).