Wipi2 (NM_178398) Mouse Untagged Clone

CAT#: MC215915

Wipi2 (untagged) - Mouse WD repeat domain, phosphoinositide interacting 2 (Wipi2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wipi2
• Synonyms: 1110018O08Rik; 2510001I10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215915 representing NM_178398
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTGGCGAGCCAGAGCGGAGAGGCCGGCGCCGGCCAGCTGCTGTTCGCCAACTTCAACCAGGATA
  ACACGTCCCTAGCTGTTGGTAGTAAGTCCGGGTATAAGTTTTTCTCCCTTTCTTCTGTGGATAAGCTGGA
  ACAGATCTATGAATGCACTGACACTGAAGATGTCTGCATTGTGGAGAGATTGTTCTCAAGCAGCTTGGTG
  GCCATTGTCAGCCTCAAAGCTCCCAGGAAGCTGAAGGTTTGCCATTTTAAGAAGGGAACTGAGATATGCA
  ACTACAGCTACTCCAACACTATCCTGGCTGTGAAGCTGAACAGGCAGCGGCTCATTGTGTGTCTGGAAGA
  GTCGCTCTACATACACAACATCCGGGACATGAAGGTACTTCATACCATCCGAGAGACACCCCCAAACCCT
  GCAGGCTTGTGTGCACTGTCAATAAACAATGACAACTGCTACTTGGCGTATCCAGGGAGTGCGAGCATTG
  GAGAGGTGCAGGTCTTCGACACCATTAACTTGAGAGCTGCAAACATGATCCCAGCTCATGATAGTCCCTT
  AGCAGCCCTGGCTTTTGATGCAAGTGGGACCAAGCTTGCCACTGCTTCTGAGAAGGGGACTGTGATTCGG
  GTGTTTTCCATTCCAGAGGGACAGAAGCTGTTTGAGTTCAGGAGAGGAGTGAAGAGGTGTGTGAGCATCT
  GCTCCCTGGCCTTCAGCATGGACGGCATGTTCCTCTCCGCATCCAGCAACACCGAAACAGTGCACATCTT
  CAAACTTGAGGCTGTGAGGGAAAAACCTCCGGAAGAGCCCACCACCTGGACTGGCTACTTTGGGAAGGTT
  CTCATGGCATCTACCAGCTACCTGCCCTCACAAGTGACAGAAATGTTCAACCAGGGCAGGGCCTTCGCCA
  CTGTCCGCCTGCCATTCTGTGGCCACAAAAACATCTGCTCACTAACCACAATTCAGAAGATCCCACGGTT
  GCTGGTAGGAGCATCAGATGGCTATTTGTACATGTACAACCTGGACCCCCAGGAGGGAGGCGAGTGTGCG
  CTGATGCGTCAGCACAGGCTTGATGGCAGTATGGAGACGACCAGTGAAATCGTAGACTCTGCATCTCATG
  ACTGCCCCTTAGCAACACAGACGTACGGCACAGCAGCTGCCAAAGGTGCCTATGTGCCCTCGTCCCCCAC
  AAGACTTGGGAAAGGGCAGGACGCCAACTTAGAAGCCTACACAGATGACCTGGGTGCTGTGGGTGGTGCA
  TGCCTAGAGGATGAAGCCAGCGCTCTGCGCCTGGATGAAGACAGCGAACATCCTCCCATGATTCTCCGGA
  CTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178398
• Insert Size: 1338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178398.4, NP_848485.1
• RefSeq Size: 3913 bp
• RefSeq ORF: 1338 bp
• Locus ID: 74781
• UniProt ID: Q80W47
• Cytogenetics: 5 G2
• Gene Summary: Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.[UniProtKB/Swiss-Prot Function]