Abhd4 (NM_134076) Mouse Untagged Clone

CAT#: MC207814

Abhd4 (untagged) - Mouse abhydrolase domain containing 4 (Abhd4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Abhd4
• Synonyms: 1110035H23Rik; Abh4; AI429574
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207814 representing NM_134076
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGGCTCAGCTCGACCCGGCAGGGCTTGTTTACTATGGCTGATGATCTGGAGCAGCAGCCTCAGG
  GCTGGCTGAGTAGCTGGCTCCCCACTTGGCGCCCCACTTCCATGTCTCAACTGAAGAATGTGGAAGCCAG
  GATCCTCCAGTGTCTCCAGAACAAGTTCCTGGCCCGTTATGTATCCCTCCCAAACCAGAACAAGATCTGG
  ACGGTGACTGTGAGCCCAGAACAAAAGGATCGCACCCCTCTGGTGATGGTACATGGCTTTGGGGGCGGTG
  TGGGCCTCTGGATCCTCAACATGGATTCACTGAGTGCCCGCCGCACACTTCATACCTTTGATCTGCTTGG
  TTTTGGGCGAAGCTCAAGGCCAACATTCCCAAGGGACCCAGAAGGAGCTGAAGATGAGTTTGTGGCCTCA
  ATAGAGACATGGCGGGAGACCATGGGAATCCCCACCATGATCCTCCTGGGGCACAGTTTGGGAGGATTCC
  TGGCCACTTCTTACTCTATCAAGTACCCTGAAAGAGTTAAACATCTTATCCTGGTGGATCCATGGGGCTT
  TCCCCTACGACCAACTGACCCTAGTGAGATCCGTGCACCTCCAACCTGGGTCAAGGCTGTGGCATCTGTC
  CTGGGACGTTCCAATCCACTGGCTGTTCTTCGAGTGGCTGGGCCTTGGGGGCCTGGGCTGGTGCAGAGAT
  TCCGTCCAGACTTCAAGCGCAAGTTTGCAGACTTCTTTGAGGATGACACCATCTCGGAATACATCTACCA
  CTGCAATGCACAGAATCCCAGTGGGGAAACGGCATTCAAAGCCATGATGGAGTCCTTTGGCTGGGCCCGG
  CGCCCCATGTTGGAGCGAATCCACTTAATTCGAAAAGATGTGCCCATCACCATGATCTATGGGGCCAACA
  CCTGGATAGATACCAGCACAGGGAAGAAGGTGAAGATGCAAAGGCCGGATTCCTACGTCCGAGACATGGA
  GATCGAGGGCGCATCCCACCACGTCTATGCTGACCAGCCACACATCTTCAATGCTGTGGTAGAAGAGATC
  TGCAACTCAGTTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134076
• Insert Size: 1068 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134076.2, NP_598837.2
• RefSeq Size: 2406 bp
• RefSeq ORF: 1068 bp
• Locus ID: 105501
• UniProt ID: Q8VD66
• Cytogenetics: 14 C2
• Gene Summary: Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis (PubMed:16818490, PubMed:25853435). Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains (PubMed:16818490, PubMed:25853435). Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (PubMed:16818490).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.