Sucla2 (BC052774) Mouse Untagged Clone

CAT#: MC207074

Sucla2 (untagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sucla2
• Synonyms: 4930547K18Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052774
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAAATTCTATAATCTTTTTCTGAAGCATGATGCAACTATGGTAGAAATAAACCCAATGGTAGAAG
  ATTCTGACGGAAAGGTGTTGTGCATGGATGCAAAGATCAATTTTGATTCAAATTCAGCCTACCGCCAGAA
  GAAGATCTTTGATCTTCAGGACTGGAGTCAGGAAGACGAAAGGGACAAAGAAGCAGCTAATGCAGATATT
  AACTACATCGGCCTCGATGGGAGCATAGGATGTCTAGTAAATGGTGCTGGCTTGGCTATGGCCACAATGG
  ATATAATAAAACTACACGGAGGGACTCCAGCCAACTTTCTTGATGTTGGTGGAGGCGCCACAGTCCAGCA
  AGTAACAGAAGCGTTTAAACTCATCACTTCAGATAAAAAGGTGCAGGCTATTCTGGTCAACATTTTTGGA
  GGAATCATGAGATGTGATGTTATTGCACAAGGAATAGTCATGGCAGTAAAGGATTTAGAAATCAGAATAC
  CTGTTGTGGTGCGGTTACAAGGTACACGAGTTGATGATGCTAAGGCACTAATAGCAGACAGTGGTCTTAA
  AATCCTTGCTTGTGATGATTTGGATGAAGCTGCTAAAATGGTTGTAAAGCTCTCTGAAATAGTGACCTTA
  GCCAAAGAAGCCCATGTGGATGTGAAGTTTCAATTGCCAATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052774
• Insert Size: 675 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052774, AAH52774
• RefSeq Size: 1180 bp
• RefSeq ORF: 674 bp
• Locus ID: 20916
• Cytogenetics: 14 D3
• Gene Summary: ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.[UniProtKB/Swiss-Prot Function]