Arhgef1 (NM_008488) Mouse Untagged Clone

CAT#: MC203719

Arhgef1 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), transcript variant 5, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef1
• Synonyms: Lbcl2; Lsc
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012488
  CGGACGCGTGGGCTCTGCGGCACGGGTGGGGGAACCTGGAGCTCGAGCGCGGAGGCTTCGGTTCCTGAGG CTGCAGTGGCTTGCTTCTCTTCCCATCCGACAGCCCGGCGGAGTCCTGGAGATGGGAGAAGTCGCCGGAG GGGCGGCCCCAGGGCCTCCCCGGTCTGGCCTGGTGTCCATCATCATCGGGGCGGAGGATGAGGATTTTGA GAACGAGCTGGAGGCGAACTCAGAAGATCAAAACAGCCAGTTCCAGAGCCTAGAGCAAGTGAAGCGCCGC CCTGCCCACCTCATGGCCCTCCTGCAGCATGTGGCCCTGCAGTTCGAGCCAGGACCACTGCTCTGCTGCC TGCATGCAGACATGCTGAGCTCTCTGGGCCCCAAAGAAGCCAAGAAGGCCTTCCTTGACTTCTATCACAG TTTCCTGGAGAAGACTGCGGTTCTACGGGTGCCGGTCCCTCCCAGTGTCGCTTTTGAACTTGATCGTACT CGACCTGATCTGATCTCTGAGGATGTCCAGAGGCGGTTCATACAAGAGGTGGTGCAGAGCCAGCAGGCAG CCGTGAGCCGTCAGCTAGAGGACTTCCGCTCTAAGCGGCTCATGGGCATGACGCCCTGGGAGCAGGAACT GAGCCTGCTGGAGCCCTGGATTGGGAAAGACCGAGGCAACTATGAGGCCCGGGAGCGGCATGTTGCGGAG CGGCTGCTGTCCCACCTGGAGGAGACCCAGCATACCATCTCTACAGATGAAGAGAAAAGTGCTGCTGTGG TCACTGCCATCAGCCTGTATATGCGCCACCTTGGAGTCCGGACCAAGAGTGGGGACAAGAAGTCGGGAAG GAACTTCTTCCGGAAAAAGGTGATGGGGAATCGGAGGTCAGACGAACCCCCAAAGACAAAGAAAGGGCTG AGCAGTATCCTAGATCCTGCACGTTGGAACCGGGGAGAGCCATCCGCTCCAGATTGTCGACATCTAAAGG TCGAGGCTGATGCAGAGAAGCCAGGCCCTGCAGACCGGAAGGGAGGCCTGGGTATGTCTTCTCGGGACAG GACTGTTGGGACTCCTGGACAGGACAACCCAGGAGTCTCCCTGCACCCTCTGTCTACAGACAGCGTCGAC TCCCGGGAACCAGGCGTGGATACCCCGCAGGAGCCAGGGGATACACCCCCACAGGGCCCTACCAGCCTGG AGCCCCTGGCGCCCCCAGAGAGCACAGAGGACAATGGCGAGACTGAGAGCCCTGAGCCCGGAGATGATGG GGAGCCAGGACGGTCAGGCCTGGAACTGGAACCAGAAGAACCTCCTGGGTGGAGGGAACTCGTGCCCCCA GACACCCTGCTCAGTCTGCCCAAGAGCCAAGTGAAGCGGCAAGAGGTCATCAGCGAGCTGCTCGTGACTG AGGCAGCTCACGTGCGCATGCTACGGGTACTGCATGACCTCTTCTACCAGCCCATGGCGGATGGAGGCTT CTTCCCTCTGGACGAGCTGCAGAACATCTTCCCGAGCCTGGATGAGCTCATCGAGGTGCACTCCCTGTTC CTCGATCGCTTGATGAAGCGGAGACAAGAGAGTGGCTACCTCATTGAGGAGATCGGCGATGTGCTACTGG CCCGGTTCGATGGTGCTGAGGGCTCATGGTTCCAGAAGATCTCCTCCCGCTTCTGCAGCCGCCAGTCGTT CGCTCTAGAGCAGCTCAAAGCCAAGCAGCGCAAGGAGCCTCGGTTCTGTGCCTTTGTGCAGGAAGCTGAG AGCCGCCCGAGATGCCGGCGCCTACAGTTAAAGGACATGATCCCCACTGAGATGCAGCGACTGACCAAGT ACCCACTGCTGCTACAGAGCATCGGGCAGAACACAGAGGAGTCTACAGAACGAGGGAAAGTGGAGCTTGC AGCTGAGTGCTGCCGGGAGATTCTGCACCATGTCAATCAAGCCGTCCGTGACATGGAGGACCTGCTGCGG CTCAAGGATTACCAGCGGCGCCTGGACTTGACTCACCTACGGCAGAGCAGTGACCCTATGCTGAGCGAGT TCAAGAACCTGGACATCACTAAGAAGAAGTTGGTCCATGAAGGCCCCCTCACGTGGCGAGTGACCAAAGA CAAAGCTATAGAAGTGCACGTGCTCTTGCTGGACGACCTGCTGCTGCTGCTCCAGCGCCAGGACGAGAGG CTGCTGCTCAAGTCCCACAGCCGGACGCTGACACCTACCCCCGATGGCAAGACCATGCTGCGGCCGGTGC TCCGGCTCACCTCTGCCATGACCCGAGAGGTGGCCACTGATCACAAAGCTTTCTACGTCATTTTTACCTG GGACCAGGAGGCCCAGATATATGAGCTGGTGGCACAGACATCTTCGGAACGCAAAAACTGGTGTAACCTC ATCACTGAGACTGCTGGATCCCTGAAGGTCCCTGCCCCTGCCTCCCGCCTCAAACCCCGGCCCAGCCCAA GCAGCATCCGAGAACCCCTGCTCAGCAGCTCTGAGAATGGCACTGGAGGCGCAGAGATGGCTCCAGCTGA TGCCAGGACAGAGCGGCTCCTCAATGACCTCCTGCCCTTCTGCAGACCAGGCCCAGAGGGCCAGCTTGCT GCCACAGCCCTTCAGAAAGTACTGTCCCTGAAGCAGATCCTGCTAAGCACTGAGGAAGACAGTGGAGCGG GGCCTCCCCGCGATGGGGATGGGGTGCCTGGTGGTAGGGCCCCCGGCCCAGTGCACACCCAGGAGATTGA GGAAAACTTGCTTAGCTTAGAGGTGGCCATCAGACAACTGGAGGAGTTGGAAGAGGAATTTTGTCGCCTA AGACCCCTCCTGTCCCAGCTTGGGGGGACTCTGTCCCCCAACCTGGCTGCACCTGAACGCTCTGCTCAGA CAGGCCTTTCATGAAGAGAAGAGTGGGGGAGAGGATGCAGGGGGGCCCACCCCCACCCCCACAGCTGCCA CAGCATCTCACACCCCAGAGGCCTGGAGCAGAGGGAGATTGGCTGAACTTGATAGCAGACCTGCTCGGGT CCCTGCCTCCCACTCTGGCCTCTGCCTTTCTCCCTCCTGCCTTCTGCTTGGGGAACTCAGGGCTTCACTC CCAGAGCACCCCCTTAATCCCCACTTTTCAGGCCGTCTCAGTATTGCCTATGGGGGGTCACCCTCCTTCC CCTACCCCAAGTGCCTTTGCTATGTTTTTATATCCTGGACTGGAGGTTTATTTTTAATATATATTATCTA AGGAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008488
• Insert Size: 2763 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012488, AAH12488
• RefSeq Size: 3250 bp
• RefSeq ORF: 2763 bp
• Locus ID: 16801
• UniProt ID: Q61210
• Cytogenetics: 7 A3
• Gene Summary: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. Isoform 3 and isoform 4 do not homooligomerize and show an enhanced RhoGEF activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) lacks two alternate in-frame exons in the mid coding region, compared to variant 1. The resulting isoform (d) is shorter than isoform a.