Chac1 (NM_026929) Mouse Untagged Clone

CAT#: MC202240

Chac1 (untagged) - Mouse ChaC, cation transport regulator-like 1 (E. coli) (Chac1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chac1
• Synonyms: 1810008K03Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025169 sequence for NM_026929
  CCGATCGGTGCCAGGCCAGGTGTACGCGTCCGTCGGTCCTTCCGTGCCCGTGCCGGAGACCAGTCTCGGA GGCCGCCCGGGTCCGTCCCTGCGCCCGGCACCATGAAGCAAGAGTCCGCATCCCAGAGCACTCCGCCTCC TTCACTGTCCCCTGCACCATCATCCGCGCAGCCTTTCTGGGGGGATGGCGACCCCCAAGCCCTGTGGATT TTCGGGTACGGCTCCCTAGTGTGGAAGCCGGACTTTGCCTATAGTGACAGCCGTGTGGGCTTCGTTCGTG GCTATAGCCGACGGTTCTGGCAGGGAGACACCTTCCATAGGGGCAGCGACAAGATGCCTGGCCGAGTGGT GACCCTCCTTGAAGACCATGAGGGCTGCACTTGGGGTGTGGCATACCAAGTTCGAGGGGAGCAGGTGAAC GAGGCCCTGAAGTACCTGAATGTGAGGGAAGCCGTGCTTGGTGGCTATGACACTAAGGAAGTCACCTTTT ATCCTCAAGACACCCCTGACCAACCCCTCACAGCACTGGCCTATGTGGCCACCCCACAGAACCCTGGCTA CCTGGGCCCTGCTCCTGAAGAGGTCATTGCCACACAGATCCTTGCTTGCCGAGGCTTCTCTGGTCACAAC CTTGAGTACTTATTGCGTTTGGCAGACTTCATGCAGCTCTGTGGGCCTCAGGCACAAGATGAGCACCTGG AAGCCATTGTGGACGCCGTAGGAACCCTGCTACCCTGCTCTTACCTCCCTGAGCAGCCTCTGGCACTGAC CTGAGGAGCCAAGCTCCTGCAAGAAGTGTCTGAGTGGTACTGGTGGACATCAGCATGCAGTACTTGAGAT AGACTTGATGGAGCAAGAGAGAGTTGAGAAGGCATGCTGGGTGGCCAGGGGCTTTGTGTCTTATGCCCCG CCTGTCTGCTAGCCTTCAGCTCTTCCTTCATTGACCCTTACTCACTACTTGAACCTTTATTTATTGCACC ATGTTGGTGTGGTGGGCAGGGCAGGTGGGGGACCTGCCCTGGATGTGGGCCCTGCAGTGCGGTGGCTCTG CCCTAGTCCTCTGGTATGTAACCAGAATCCCCCCATTGCTGCTGCCAATTCCACACCACCCAGGCCTCCG TAGCCCCAGGGACCCTCCAAGATCGTTGCTCCTCTGTCCACCCAGACTACCCCAGCCGTGGTAGTATCAC CTGCCCATGTTCCGGGCCTTCTGGATGAGGGACAGCAGAGAAGGAGGCGAAGTAGCTCTTCAAGGGCCAA GGGCCAGCATCCCTCTTCATTGACGGGCCCGGGGCAGAGCCGGATCCTATGCACAGACACAGTTCCTGGG ACTGGGCCTCTCCCCCATCCCTCTTTTTTCATTTCCTTATCCTTTTGTCTGTCTGCTTGTTTGTCTGGAT CACTTCCTATAGACCCATTGGTCTGTTCCTGGAAAACACTACAGTCCTTGTCCATTCTCAGTTGACCCAT ACTGTTACCCATAAACTGTCTTTCATTAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_026929
• Insert Size: 672 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025169, AAH25169
• RefSeq Size: 1514 bp
• RefSeq ORF: 672 bp
• Locus ID: 69065
• UniProt ID: Q8R3J5
• Cytogenetics: 2 E5
• Gene Summary: Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (By similarity). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).[UniProtKB/Swiss-Prot Function]