Map3k20 (NM_023057) Mouse Untagged Clone

CAT#: MC201671

B230120H23Rik (untagged) - Mouse RIKEN cDNA B230120H23 gene (B230120H23Rik), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map3k20
• Synonyms: AV006891; B230120H23Rik; MLTK; MLTKalpha; MLTKbeta; Zak
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023718 sequence for NM_023057
  CCACGCGTCCGTTCAACTCCTGACTCCGGCGGAAGCGTGGGAGCCGCGCGGGCCGCCGTCCCCGACCCCC GCCGCCGCTCGCGCCCGATCCGGGAGCCAGATTTTGTGGACGTTTACTACTTTGTCATTATGAGATGTCG TCTCTCGGAGCCTCCTTTGTGCAAATTAAGTTCGACGACTTGCAGTTTTTTGAAAACTGCGGTGGAGGAA GTTTTGGGAGTGTGTATCGAGCCAAATGGATATCACAGGACAAGGAGGTGGCTGTAAAGAAGTTACTCAA AATAGAGAAAGAGGCAGAAATCCTGAGCGTCCTCAGTCACAGAAACATCATCCAGTTTTATGGAGTGATT CTGGAACCTCCCAACTATGGCATCGTCACAGAATATGCTTCCCTGGGCTCCCTGTATGATTACATTAACA GCAACAGGAGTGAAGAGATGGACATGGAACACATCATGACCTGGGCCACTGACGTAGCCAAAGGGATGCA TTACTTACACATGGAAGCTCCTGTCAAGGTGATCCACAGAGACCTCAAGTCAAGAAACGTTGTTATCGCT GCTGACGGAGTGCTGAAGATCTGTGACTTCGGTGCCTCGCGGTTCCATAACCACACAACACACATGTCCT TGGTTGGAACTTTCCCATGGATGGCTCCAGAAGTTATCCAGAGTCTCCCTGTGTCTGAAACCTGTGACAC GTATTCCTATGGTGTGGTTCTCTGGGAGATGCTAACAAGGGAGGTCCCCTTTAAAGGTTTGGAAGGATTA CAAGTAGCTTGGCTTGTAGTGGAAAAAAACGAGAGATTAACCATTCCAAGCAGCTGCCCCAGAAGCTTTG CTGAACTGCTACACCAGTGTTGGGAGGCTGATGCCAAGAAACGGCCATCGTTCAAGCAAATCATTTCAAT CCTGGAGTCTATGTCAAATGACACGAACCTTCCTGACCAGTGTAACTCATTTTTACACAACAAGGCGGAG TGGAGGTGTGAAATTGAGGCAACCCTTGAGCGACTGAAGAAACTAGAGCGAGATCTCAGCTTTAAAGAGC AGGAGCTCAAAGAACGGGAGAGACGTCTCAAGATGTGGGAGCAGAAGCTGACGGAGCAATCCAACACCCC GCTGCTGCCTTCCTTTGAGATTGGTGCGTGGACTGAAGACGATGTGTATTTTTGGGTTCAGCAGCTCGTC AGAAAAGGCGAGTCTTCAGTAGAGATGAGTGGATACGCAAGTTTGTTTAAGGAGAACAACATCACAGGCA AGCGGTTGCTGCTTCTGGAAGAGGAAGATCTGAAAGACATGGGCATCGTCTCCAAGGGACACATCATTCA CTTTAAGTCAGCTATTGAGAAGCTAACCCACGATTATCTGAACCTGTTTCACTTCCCACCACTGATTAAG GATTCAGGAGGGGAACCTGAAGAAAATGAGGAAAAAATAGTGAACCTTGAACTTGTTTTTGGTTTTCACT TGAAGCCAGGAACTGGCCCACAGGATTGCAAATGGAAAATGTATATGGAGATGGATGGGGACGAAGTTGC AATCACGTACATAAAAGATGTGACTTTCAACACGAGCCTCCCTGATGCAGAGATTTTAAAGATGACAAAG CCACCATTTGTAATGGAGAAGTGGATCGTGGGAATAGCGGAGGATCAGACTGTGGAGTGCACGGTCACCT ATGAGAATGATGTCAGAACACCAAAACTCACTAAGCACGTGCACTCCATACAGTGGGACAGAACGAAGCC TCAGGATGAGGTGAAGGCGGTCCAGCTTGCCATTCAGACTCTGTTCTCCAGCTCAGAGGGCAACCCTGGC AGCAGATCCGACTCCAGTGCGGACTGCCAATGGTTAGACACTCTGCGCATGCGGCAGATTGCATCCCACA CTTCTCTACAGCGTTCCCAAAGCAACCCTATCTTGGGGTCTCCTTTCTTCCCTTACTTTGCCAACCAGGA TTCCTATGCTGCTGCGGTGAGGAGGACCCAGACTCCGGTGAAGTACCAGCAGATTACACCAAGCATCAAC CCTTCCCGGAGCTCCTCCCCCACGCAGTATGGGCTGTCCAGAAACTTCTCCTCCCTGAACCTCAGCTCCA GGGACAGCGGCTTCTCCAGCCTCAATGACAGCTCCTCCGAGAGGGGCCGCTACTCAGACCGAAGCAGAAA CAAGTACTACCGTGGTAGTGTGTCCCTCAATTCTTCCCCGAAAGGAAGATATGGTGGGAAAAGTCAGCAT TCCACACCTTCAAGAGAAAGATATTCTGGAAAGTTCTACAGGCTGCCCCAGTCAGCGCTGAACACTCATC AGTCCCCTGACTTCAAGAGGAGCCCAAATGACCATGACCGCCGTGTGCCCAGGACCATACCCGGGATGCC TCTGCACCCTGAGACTGCCTCCAAAGCAGGGGAAGAGGAGAGCAGGGTGAGCGAAGGGGGCTGGACTAAA GTGGAATATCGGAAGAAGACACACAGGCAACTTTCGGCCAAGACTAGCAAGGAGAGAACCCGTGGCAACT ACCGTGGGCGGCGGAATTTTTGAGGGATTGGGTCAGATGCGCTTTTCCAAGCGGGTTAGTAGTGCTTTGT GCAATGGATTTTTGGTAATAAGGTATCTTGAGGTTATAGGGACCAAAAAAAGACTAGTTCTGGGCTTGGG AAACACCTTTTAACTGAAAGGACAACCAAAGCAGGGCCTCAATTATGGCTGTTAGTCAGTCAACTAGAGG TGGCAATTAAACTTGACAATAACTGCCAATGTTTTGTCCCCTGAATTACTATTGAGGTGGGGAGGGCTAT GACATTGCCTGCTGGGAACATTCGAGCAGGCTTGGGGCCAGGATAAAGCTAGTGAGAACCACAGAAAAAT CATATTTCTGGACAAAACAGGATTCATCTTATCTTCTCTTTCATCTAAGTTCCAAGATGGCTCTGCAAAG CACTGTGGACGATTCTTGAGTTAAAATTTGTTTTTTGATTTTGGGGTTTTGTTTTTTTTTTTTTAACCTA CTGGGATAAAATATCATTTATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023057
• Insert Size: 2409 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023718, AAH23718
• RefSeq Size: 3079 bp
• RefSeq ORF: 2409 bp
• Locus ID: 65964
• UniProt ID: Q9ESL4
• Cytogenetics: 2 C3
• Gene Summary: Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways (PubMed:11042189). Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14 (By similarity). Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2 (By similarity). Involved in limb development (PubMed:26755636).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) is the longest transcript and encodes the longest isoform (1).