Polr3f (NM_029763) Mouse Untagged Clone

CAT#: MC200703

Polr3f (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide F (Polr3f), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Polr3f
• Synonyms: 2810411G20Rik; 3010019O03Rik; 3110032A07Rik; RPC6; RPC39
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC009159 sequence for NM_029763
  CTCGCCTCTCTCTCAAGCCTCGGTCCCCGGCGCGCGCCTCCACCGGTTCCCGAGACTTCCAGCGCGCTCC GAGGGGCCACTTTTCCTCTAGTCTCGAAAACTGCCCTCATGGCGGAGGTGAAGGTGAAGGTGCAGCCGCC GGACGCGGATCCGGTGGAGATAGAAAACAGAATTATAGAATTATGCCACCAGTTCCCTCATGGAATCACA GATCAAGTAATTCAGAACGAAATGCCTCACATTGAAGCCCAGCAGCGGGCAGTGGCCATCAATAGACTGT TATCCATGGGTCAGTTGGATCTTTTAAGGAGTAATACAGGCCTCTTATATAGAATAAAGGATTCTCAGAA TGCTGGTAAAATGAAGGGGTCCGATAACCAGGAAAAACTGGTCTATCAAATCATAGAGGATGCAGGGAAT AAAGGAATATGGAGCAGAGATATTCGGTACAAAAGTAACTTGCCGTTAACAGAAATCAACAAAATTCTCA AGAATTTGGAAAGTAAAAAGCTAATTAAAGCCGTGAAGTCTGTAGCAGCTTCCAAAAAGAAGGTTTATAT GCTCTATAACCTGCAGCCAGACCGATCTGTGACTGGCGGTGCCTGGTACAGTGACCAGGATTTTGAATCT GAATTTGTTGAGGTGCTTAATCAACAGTGTTTCAAATTCCTACAAAGCAAGGCAGAGACAGCAAGAGAAA GTAAACAGAATCCAGTGATACAAAGAAATAGTTCATTTGCATCGTCACATGAAGTCTGGAAGTACATCTG TGAGCTGGGCATCAGCAAGGTAGAGTTGTCCATGGAGGACATTGAAACCATCTTGAACACACTCATTTAT GATGGCAAAGTGGAGATGACTATCATTGCTGCGAAGGAAGGCACTGTTGGCAGCGTAGATGGACACATGA AACTGTACAGGGCAGTCAACCCAATCCTCCCACCTACAGGCGTGGTCCGGGCACCCTGTGGACTCTGCCC CGTTTTTGAGGACTGCCATGAAGGTGGTGAGATTTCGCCGTCTAATTGTATTTACATGACCGAGTGGCTT GAATTTTAATGAGAGCTGTGCACTTTATCTACATTTGGCAAACTGAACTCCATGGAGAACAAACAATTCA TGATGGAGTGTAAATTTCTTTGAAAGCATACTTCACAATAATGGATACTGACTTGCTGCTATGAAAACAT TTATTTATGAAGACAGAACACAAAGCAGATTGAGTTTAATAAAGTTTAATTGGGAAGTCTCTTCTTCAGT AAGTAAAATACCTTGAAGGTCTGGAAGGCAAGGAAAAGAAGCCAGCAAGCCTATGTTGACCAAGTACTGA GATAAAGGCTGGAACGAGAAGATGTTCACTAGAGTTGCTTCATTGAAAACTCATCGGGGTTATTACCACC ATTTGTTTGGTTGTTTTTGGGTTTTGTTGAGAAGAAATTACAAGTGGTGCAGATAGGAACAGCTGATGGC ATGTGTGCCATCTTGGGGCCTGGGTAGTCAAGTGGGCATTCCTTTTTCATGCCATCTGTTGCTCTTCACC CCAGGACTCTTTCAGGGGAGTGTGCCTCCATACCGACCTCACTCCCAAATGCTGTTGTACAGGACGTGTT TCCACATCACTGTTATTCCATGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_029763
• Insert Size: 951 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009159, AAH09159
• RefSeq Size: 1665 bp
• RefSeq ORF: 951 bp
• Locus ID: 70408
• UniProt ID: Q921X6
• Cytogenetics: 2 G1
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity). Preferentially binds double-stranded DNA (dsDNA) (By similarity).[UniProtKB/Swiss-Prot Function]