Human EIF5 (NM_183004) AAV Particle

CAT#: RC213587A1V

AAV ORF Particles, serotype AAV-2, EIF5 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: EIF5
• Synonyms: EIF-5; EIF-5A
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213587 representing NM_183004
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTCAATGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATGCCCCGTCTGATTGCCA
  AGGTTGAGGGCAAAGGCAATGGAATCAAGACAGTTATAGTCAACATGGTTGACGTTGCAAAGGCGCTTAA
  TCGGCCTCCAACGTATCCCACCAAATATTTTGGTTGTGAGCTGGGAGCACAGACCCAGTTTGATGTTAAG
  AATGACCGTTACATTGTCAATGGATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTCATTA
  AAAAATTTGTTCTCTGTCCTGAATGTGAGAATCCTGAAACAGATTTGCATGTCAATCCAAAGAAGCAAAC
  AATAGGTAATTCTTGTAAAGCCTGTGGCTATCGAGGCATGCTTGACACACATCATAAACTCTGCACATTC
  ATTCTCAAAAACCCACCTGAGAATAGTGACAGTGGTACAGGAAAGAAAGAAAAAGAAAAGAAAAACAGAA
  AGGGCAAAGACAAGGAAAATGGCTCCGTATCCAGCAGTGAGACACCACCACCACCACCACCACCAAATGA
  AATTAATCCTCCTCCACATACAATGGAAGAAGAGGAGGATGATGACTGGGGAGAAGATACAACTGAGGAA
  GCTCAAAGGCGTCGAATGGATGAAATCAGTGACCATGCAAAAGTTCTGACACTCAGTGATGATTTGGAAA
  GAACAATTGAGGAGAGGGTCAATATCCTCTTTGATTTTGTTAAGAAAAAGAAAGAAGAGGGTGTTATTGA
  TTCATCTGACAAAGAAATCGTTGCTGAAGCAGAAAGACTGGATGTAAAAGCCATGGGCCCTCTTGTTCTA
  ACTGAAGTTCTTTTTAATGAGAAGATTAGAGAACAGATTAAGAAATACAGGCGCCATTTCCTACGATTTT
  GTCACAACAACAAAAAAGCCCAACGGTACCTTCTTCATGGTTTGGAGTGTGTGGTAGCAATGCATCAAGC
  TCAGCTTATCTCCAAGATTCCACATATCTTGAAGGAGATGTACGATGCAGACCTTTTAGAAGAAGAGGTC
  ATCATCAGCTGGTCGGAAAAGGCCTCTAAGAAATATGTCTCCAAAGAACTTGCCAAAGAGATTCGTGTCA
  AAGCAGAACCATTTATAAAATGGTTGAAGGAGGCAGAGGAAGAATCTTCTGGTGGCGAAGAAGAAGATGA
  AGATGAGAACATTGAGGTGGTGTATTCGAAGGCTGCCAGTGTACCGAAAGTTGAGACTGTAAAGTCAGAC
  AACAAGGATGACGACATCGATATTGATGCCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213587 representing NM_183004
  Red=Cloning site Green=Tags(s)
  
  MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK
  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTF
  ILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEE
  AQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVL
  TEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEV
  IISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSD
  NKDDDIDIDAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_183004
• ORF Size: 1293 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_183004.3
• RefSeq Size: 5782 bp
• RefSeq ORF: 1296 bp
• Locus ID: 1983
• UniProt ID: P55010
• Cytogenetics: 14q32.32
• MW: 49 kDa