Human CYB5R4 (NM_016230) AAV Particle

CAT#: RC205203A1V

AAV ORF Particles, serotype AAV-2, CYB5R4 (Myc-DDK-tagged)-Human cytochrome b5 reductase 4 (CYB5R4), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: CYB5R4
• Synonyms: cb5/cb5R; dJ676J13.1; NCB5OR
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205203 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAACGTCCCTTCCCAGTCTTTCCCGGCCCCCAGGTCGCAGCAGCGTGTCGCCTCCGGGGGGCGTA
  GCAAGGTACCTTTAAAACAGGGCAGAAGCCTTATGGATTGGATTCGACTGACCAAAAGTGGAAAGGATCT
  AACGGGATTAAAAGGCAGGTTAATTGAAGTAACTGAAGAAGAACTTAAGAAACACAACAAAAAAGATGAT
  TGTTGGATATGCATAAGAGGTTTCGTTTATAATGTCAGCCCTTATATGGAGTATCATCCTGGTGGAGAAG
  ATGAACTAATGAGAGCAGCAGGATCAGATGGTACTGAACTTTTTGATCAGGTTCATCGTTGGGTCAATTA
  TGAATCCATGCTGAAAGAATGCCTGGTTGGCAGAATGGCCATTAAACCTGCTGTTCTGAAAGACTATCGT
  GAGGAGGAAAAGAAAGTCTTAAATGGCATGCTTCCCAAGAGCCAAGTGACAGATACACTTGCCAAAGAAG
  GTCCTAGTTATCCAAGCTATGATTGGTTCCAAACAGACTCTTTAGTCACCATTGCCATATATACTAAACA
  GAAGGATATCAATTTAGACTCAATAATAGTTGATCATCAGAATGATTCCTTTAGAGCAGAAACAATTATT
  AAGGATTGTTTATATCTTATACATATTGGGCTAAGCCATGAGGTTCAGGAAGATTTTTCTGTGCGGGTTG
  TTGAGAGTGTGGGAAAAATAGAGATTGTTCTACAAAAAAAAGAGAATACTTCTTGGGACTTTCTTGGCCA
  TCCCCTGAAGAATCATAATTCACTTATTCCAAGGAAAGATACAGGTTTGTACTACAGAAAGTGCCAGTTA
  ATTTCCAAGGAAGATGTTACTCATGATACGAGGCTTTTCTGTTTGATGCTGCCACCAAGCACTCATCTTC
  AAGTGCCCATTGGGCAACATGTTTACCTCAAGCTACCTATTACAGGTACAGAAATAGTAAAGCCATATAC
  ACCTGTATCTGGTTCCTTACTCTCAGAGTTCAAGGAACCAGTTCTTCCCAACAATAAATACATCTACTTT
  TTGATAAAAATCTATCCCACTGGACTCTTCACACCAGAGCTTGATCGTCTTCAGATTGGAGATTTTGTTT
  CTGTAAGCAGTCCTGAGGGCAATTTTAAAATATCCAAGTTCCAAGAATTAGAAGATCTCTTTTTGTTGGC
  AGCTGGAACAGGCTTCACACCAATGGTTAAAATACTGAATTATGCTTTGACTGATATACCCAGTCTCAGG
  AAAGTGAAGCTGATGTTCTTCAATAAAACAGAAGATGATATAATTTGGAGAAGCCAATTGGAGAAATTAG
  CATTTAAAGATAAAAGACTGGATGTTGAATTTGTTCTCTCAGCACCTATTTCTGAATGGAATGGCAAACA
  GGGACATATTTCACCAGCTCTTCTTTCTGAATTTTTGAAAAGAAATTTGGACAAATCCAAAGTTCTCGTC
  TGCATTTGTGGACCAGTGCCATTTACAGAACAAGGAGTAAGGTTGCTGCATGATCTCAACTTTTCCAAAA
  ATGAGATCCATAGTTTTACAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205203 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLNVPSQSFPAPRSQQRVASGGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGRLIEVTEEELKKHNKKDD
  CWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESMLKECLVGRMAIKPAVLKDYR
  EEEKKVLNGMLPKSQVTDTLAKEGPSYPSYDWFQTDSLVTIAIYTKQKDINLDSIIVDHQNDSFRAETII
  KDCLYLIHIGLSHEVQEDFSVRVVESVGKIEIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYRKCQL
  ISKEDVTHDTRLFCLMLPPSTHLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLLSEFKEPVLPNNKYIYF
  LIKIYPTGLFTPELDRLQIGDFVSVSSPEGNFKISKFQELEDLFLLAAGTGFTPMVKILNYALTDIPSLR
  KVKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLKRNLDKSKVLV
  CICGPVPFTEQGVRLLHDLNFSKNEIHSFTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_016230
• ORF Size: 1563 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_016230.3
• RefSeq Size: 2259 bp
• RefSeq ORF: 1566 bp
• Locus ID: 51167
• UniProt ID: Q7L1T6
• Cytogenetics: 6q14.2
• MW: 59.5 kDa