Mouse Chrna2 (NM_144803) AAV Particle

CAT#: MR208228A1V

AAV ORF Particles, serotype AAV-2, Chrna2 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) (Chrna2), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Chrna2
• Synonyms: Acra-2; Acra2; BC011490
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208228 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCTCCCATCCTGCTTTCCAGTTCTGGATACATCTTTATCTCTGGTGTCTGCTTCTGATGCCAG
  CAGTGTTGGCCCAGCAAGGCTCGCACACCCATGCCGAGGACCGCCTGTTCAAACACCTCTTTGGAGGCTA
  CAATCGCTGGGCACGGCCAGTGCCCAACACTTCCGATGTGGTCATCGTGCGCTTTGGACTATCCATTGCA
  CAGCTCATAGATGTGGATGAGAAGAATCAAATGATGACCACCAATGTCTGGCTAAAGCAGGAATGGAATG
  ACTACAAGCTGCGCTGGGACCCGGCTGAGTTTGGCAACATCACCTCCCTCCGCGTCCCCTCAGAGATGAT
  CTGGATCCCAGACATTGTTCTCTACAACAATGCAGACGGGGAGTTTGCGGTGACCCACATGACCAAGGCC
  CACCTCTTCTTCACGGGCACTGTGCACTGGGTGCCCCCAGCCATCTACAAGAGCTCCTGCAGCATCGACG
  TGACTTTCTTCCCCTTCGACCAGCAGAACTGCAAGATGAAGTTTGGCTCCTGGACATACGACAAGGCCAA
  GATCGACCTGGAGCAGATGGAGCGGACGGTGGACCTGAAGGACTACTGGGAGAGCGGCGAGTGGGCCATT
  ATCAACGCCACCGGAACCTATAACAGCAAGAAGTACGACTGCTGCGCGGAGATCTACCCCGACGTCACCT
  ACTACTTCGTTATCCGCCGGCTGCCGCTGTTCTACACCATCAACCTCATTATCCCGTGCCTGCTCATCTC
  CTGCCTCACCGTGCTCGTGTTCTACTTGCCCTCTGAGTGTGGAGAGAAGATCACGCTGTGCATCTCGGTG
  CTGCTCTCCCTCACTGTCTTCCTGCTGCTCATCACAGAAATTATCCCATCCACCTCACTGGTCATCCCAC
  TCATCGGCGAGTACCTGCTCTTCACCATGATCTTCGTCACCCTCTCTATTGTGATCACAGTCTTCGTGCT
  CAATGTCCACCACCGTTCCCCTAGCACGCACAACATGCCCAACTGGGTGAGGGTGGCCCTGCTAGGCCGG
  GTGCCCCGGTGGCTGATGATGAATCGGCCCCTGCCGCCCATGGAGCTCCATGGCTCTCCAGGTCTGAAGC
  TCAGCCCCACTTATCATTGGCTGGAGACCAACATGGATGCTGAAGAAAGGGAGGAGACAGAGGAAGAGGA
  GGAGGAGGAAGAAGATGAAAACATATGTATGTGTGCAGGCCTCCCAGACTCTTCCATGGGTGTCCTCTAT
  GGCCATGGCAGCCTGCATCTGAGGGCCATGGGGCCTGAGGCCAAGACTCCATCCCAGGCTAGTGAGATCC
  TGCTGTCACCTCAAATACAGAAAGCACTAGAAGGTGTACACTACATTGCTGACCACCTGAGGTCTGAGGA
  TGCTGACTCTTCGGTGAAGGAAGATTGGAAGTATGTGGCCATGGTGGTAGACCGGATCTTCCTCTGGCTG
  TTCATTATCGTCTGCTTCCTGGGGACCATCGGGCTCTTCCTCCCTCCGTTCCTGGCTGGAATGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208228 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPSHPAFQFWIHLYLWCLLLMPAVLAQQGSHTHAEDRLFKHLFGGYNRWARPVPNTSDVVIVRFGLSIA
  QLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKA
  HLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMERTVDLKDYWESGEWAI
  INATGTYNSKKYDCCAEIYPDVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISV
  LLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHNMPNWVRVALLGR
  VPRWLMMNRPLPPMELHGSPGLKLSPTYHWLETNMDAEEREETEEEEEEEEDENICMCAGLPDSSMGVLY
  GHGSLHLRAMGPEAKTPSQASEILLSPQIQKALEGVHYIADHLRSEDADSSVKEDWKYVAMVVDRIFLWL
  FIIVCFLGTIGLFLPPFLAGMI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_144803
• ORF Size: 1539 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_144803.2, NP_659052.1
• RefSeq Size: 3527 bp
• RefSeq ORF: 1539 bp
• Locus ID: 110902
• UniProt ID: Q91X60
• Cytogenetics: 14 34.36 cM
• MW: 58.7 kDa