Mouse Mthfd2 (NM_008638) AAV Particle

CAT#: MR205312A1V

AAV ORF Particles, serotype AAV-2, Mthfd2 (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase (Mthfd2), nuclear gene encoding mitochondrial protein, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Mthfd2
• Synonyms: AW558851; NMDMC
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205312 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCAGTTTCCTTGTTGTCTGCGTTGGCTGTGCGGTTGTTGCGCCCCACGCACGGCTGCCACCCCC
  GCCTACAGCCCTTCCACCTGGCGGCAGTGCGAAATGAAGCCGTTGTCATTTCTGGAAGGAAACTGGCCCA
  GCAGATCAAGCAAGAAGTACAGCAGGAGGTGGAAGAGTGGGTGGCCTCAGGCAATAAGCGGCCACACCTC
  AGTGTCATTCTGGTTGGTGACAATCCCGCCAGTCACTCCTATGTTCTCAACAAAACCAGGGCAGCAGCTG
  AAGTGGGAATCAACAGTGAGACAATTGTGAAACCAGCCTCAGTTTCAGAGGAAGAGCTGCTGAATTCAAT
  CAGGAAGCTGAACAACGATGAGAACGTAGATGGCCTCCTTGTTCAGCTGCCACTCCCAGAGCACATTGAT
  GAGAGGAAGGTCTGCAATGCCGTTTCTCCTGACAAGGATGTTGATGGCTTTCATGTCATTAACGTGGGGC
  GAATGTGCTTGGACCAGTACTCTATGCTGCCAGCAACCCCATGGGGCGTGTGGGAGATAATTAAGCGAAC
  AGGCATTCCAACCTTAGGGAAGAATGTGGTAGTGGCTGGCAGGTCAAAAAACGTTGGAATGCCGATTGCA
  ATGTTGCTGCACACAGATGGAGCTCACGAACGGCCCGGGGGTGATGCCACAGTCACAATATCTCATCGAT
  ACACTCCCAAAGAGCAGCTGAAGAAGCACACGATCCTTGCAGACATTGTAATATCTGCCGCTGGCATTCC
  AAATCTGATCACAGCTGACATGATCAAGGAAGGAGCAGCGGTCATCGATGTGGGAATAAACAGAGTTCAA
  GATCCTGTCACTGCAAAGCCCAAATTGGTTGGAGATGTGGATTTTGAAGGAGTCAAGAAGAAAGCTGGTT
  ATATCACTCCCGTCCCTGGTGGTGTGGGTCCCATGACCGTGGCCATGCTCATGAAGAACACCATTATTGC
  CGCAAAGAAAGTGCTAAGACCAGAGGAGCTGGAAGTGTTCAAGTCCAAACAGCGCGGAGTCGCCACCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205312 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASVSLLSALAVRLLRPTHGCHPRLQPFHLAAVRNEAVVISGRKLAQQIKQEVQQEVEEWVASGNKRPHL
  SVILVGDNPASHSYVLNKTRAAAEVGINSETIVKPASVSEEELLNSIRKLNNDENVDGLLVQLPLPEHID
  ERKVCNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIA
  MLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVQ
  DPVTAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRPEELEVFKSKQRGVATN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_008638
• ORF Size: 1053 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_008638.1, NP_032664.1
• RefSeq Size: 2070 bp
• RefSeq ORF: 1053 bp
• Locus ID: 17768
• UniProt ID: P18155
• Cytogenetics: 6 35.94 cM
• MW: 37.9 kDa