Mouse Rfc5 (NM_028128) AAV Particle

CAT#: MR205065A1V

AAV ORF Particles, serotype AAV-2, Rfc5 (Myc-DDK-tagged) - Mouse replication factor C (activator 1) 5 (Rfc5), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Rfc5
• Synonyms: 36.5kDa; 36kDa; 2610020K06Rik; 2610209F07Rik; Recc5
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205065 representing NM_028128
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCGGCCGCGCCTTCGCAGCAGCGGCCCGCGGCGGCCCGGGCCCGGAACCTGCCCTGGGTTGAGA
  AGTACCGGCCACAGACCCTGGCCGATCTCATTTCTCACCAGGACATTCTGAGTACCATTCAGAAGTTCAT
  CAGTGAAGACCGCCTGCCACACCTACTTCTCTATGGCCCTCCAGGGACAGGAAAGACATCCACCATCCTG
  GCCTGTGCCAAGCAACTGTACAAAGATAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCTGACG
  ACCGAGGGATCGATATTGTTCGGGGGCCAATCCTCAGCTTTGCCAGCACAAGGACAATCTTCAAGAAAGG
  GTTTAAGCTTGTGATCCTGGACGAAGCCGACGCCATGACTCAGGATGCCCAGAACGCCTTGAGACGAGTG
  ATTGAGAAGTTCACAGAAAACACCAGGTTCTGCCTCATCTGTAACTACCTGTCCAAGATCATCCCTGCCT
  TGCAGTCTCGGTGCACGAGGTTCCGATTTGGCCCTCTGACACCCGAGCTCATGGTCCCTCGACTGGAACA
  TGTAGTACAAGAAGAGAATGTGGATATAAGTGAAGATGGAATGAAGGCCCTTGTCACTCTGTCCAGTGGG
  GACATGCGAAGGGCTTTGAACATCCTTCAGAGTACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTG
  TCTACACCTGCACCGGACACCCACTCAAGACGGACATTGCCAATATACTGGACTGGATGCTCAATCAGGA
  CTTCACCACTGCCTACAAAAACATCATGGAGCTGAAGACTCTGAAGGGCTTGGCGCTGCATGACATCCTG
  ACAGAGGTTCACTTGTTTGTACACAGAGTTGACTTTCCATCTTCAGTTCGGATACACTTATTGACCAAAA
  TGGCAGACATCGAGTACAGACTTTCTGTTGGCACCAGTGAGAAGATACAGCTGAGCTCCCTCATCGCCGC
  TTTTCAAGTCACCAGAGACCTGATAGTTGCAGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205065 representing NM_028128
  Red=Cloning site Green=Tags(s)
  
  MTAAAPSQQRPAAARARNLPWVEKYRPQTLADLISHQDILSTIQKFISEDRLPHLLLYGPPGTGKTSTIL
  ACAKQLYKDKEFGSMVLELNASDDRGIDIVRGPILSFASTRTIFKKGFKLVILDEADAMTQDAQNALRRV
  IEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMVPRLEHVVQEENVDISEDGMKALVTLSSG
  DMRRALNILQSTNMAFGKVTEETVYTCTGHPLKTDIANILDWMLNQDFTTAYKNIMELKTLKGLALHDIL
  TEVHLFVHRVDFPSSVRIHLLTKMADIEYRLSVGTSEKIQLSSLIAAFQVTRDLIVAEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_028128
• ORF Size: 1017 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_028128.1, NP_082404.1
• RefSeq Size: 1750 bp
• RefSeq ORF: 1020 bp
• Locus ID: 72151
• UniProt ID: Q9D0F6
• Cytogenetics: 5 F
• MW: 38.5 kDa