Deleted in azoospermia 4 (DAZ4) (NM_020420) Human 3' UTR Clone

CAT#: SC216897

3' UTR clone of deleted in azoospermia 4 (DAZ4) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DAZ4
• Synonyms: pDP1680; pDP1681
• ACCN: NM_020420
• Insert Size: 1922 bp
• Sequence Data:
  Insert Sequence

  >SC216897 3’UTR clone of NM_020420
  The sequence shown below is from the reference sequence of NM_020420. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTTGTCTGTTTAATCCAGAGAAGAGACTGATAAATTCCGTTGTTACTCAAGATGACTGCTTCAAGGGTA
  AAAGAGTGCATCGCTTTAGAAGAAGTTTGGCAGTATTTAAATCTGTTGGATCCTCTCAGCTATCTAGTT
  TCATGGGAAGTTGCTGGTTTTGAATATTAAGCTAAAAGTTTTCCACTATTACAGAAATTCTGAATTTTG
  GTAAATCACACTGAAACTTTCTGTATAACTTGTATTATTAGACTCTCTAGTTTTATCTTAACACTGAAA
  CTGTTCTTCATTAGATGTTTATTTAGAACCTGGTTCTGTGTTTAATATATAGTTTAAAGTAACAAATAA
  TCGAGACTGAAAGAATGTTAAGATTTATCTGCAAGGATTTTTAAAAAATTGAAACTTGCATTTTAAGTG
  TTTAAAAGCAAATACTGACTTTCAAAAAAGTTTTTAAAACCTGATTTGAAAGCTAACAATTTTGATAGT
  CTGAACACAAGCATTTCACTTCTCCAAGAAGTACCTGTGAACAGTACAATATTTCAGTATTGAGCTTTG
  CATTTATGATTTATCTAGAAATTTACCTCAAAAGCAGAATTTTTAAAACTGCATTTTTAATCAGTGGAA
  CTCAATGTATAGTTAGCTTTATTGAAGTCTTATCCAAACCCAGTAAAACAGATTCTAAGCAAACAGTCC
  AATCAGTGAGTCATAATGTTTATTCAAAGTATTTTATCTTTTATCTAGAATCCACATATGTATGTCCAA
  TTTGATTGGGATAGTAGTTAGGATAACTAAAATTCTGGGCCTAATTTTTTAAAGAATCCAAGACAAACT
  AAACTTTACTGGGTATATAACCTTCTCAATGAGTTACCATTCTTTTTTATAAAAAAAATTGTTCCTTGA
  AATGCTAAACTTAATGGCTGTATGTGAAATTTGCAAAATACTGGTATTAAAGAACGCTGCAGCTTTTTT
  ATGTCACTCAAAGGTTAATCGGAGTATCTGAAAGGAATTGTTTTTATAAAAACATTGAAGTATTAGTTA
  CTTGCTATAAATAGATTTTTATTTTTGTTTTTTAGCCTGTTATATTTCCTTCTGTAAAATAAAATATGT
  CCAGAAGAGGCATGTTGTTTCTAGATTAGGTAGTGTCCTCATTTTATATTGTGACCACACAGCTAGAGC
  ACCAGAGCCCTTTTGCTATACTCACAGTCTTGTTTTCCCAGCCTCTTTTACTAGTCTTTCAGGAGGTTT
  GCTCTTAGAACTGGTGATGTAAAGAATGGAAGTAGCTGTATGAGCAGTTCAAAGGCCAAGCCGTGGAAT
  GGTAGCAATGGGATATAATACCTTTCTAAGGGAAACATTTGTATCAGTATCATTTGATCTGCCATGGAC
  ATGTGTTTAAAGTGGCTTTCTGGCCCTTCTTTCAATGGCTTCTTCCCTAAAACGTGGAGACTCTAAGTT
  AATGTCGTTACTATGGGCCATATTACTAATGCCCACTGGGGTCTATGATTTCTCAAAATTTTCATTCGG
  AATCCGAAGGATACAGTCTTTAAACTTTAGAATTCCCAAGAAGGCTTTATTACACCTCAGAAATTGAAA
  GCACCATGACTTTGTCCATTAAAAAATTATCCATAGTTTTTTTAGTGCTTTTAACATTCCGACATACAT
  CATTCTGTGATTAAATCTCCAGATTTCTGTAAATGATACCTACATTCTAAAGAGTTAATTCTAATTATT
  CCGATATGACCTTAAGGAAAAGTAAAGGAATAAATTTTTGTCTTTGTTGAAGTATTTAATAGAGTAAGG
  TAAAGAAGATATTAAGTCCCTTTCAAAATGGAAAATTAATTCTAAACTGAGAAAAATGTTCCTACTACC
  TATTGCTGATACTGTCTTTGCATAAATGAATAAAAATAAACTTTTTTTCTTCAAATGTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_020420.4
• Summary: This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2011]
• Locus ID: 57135
• MW: 74.8