Deleted in azoospermia 4 (DAZ4) (NM_020420) Human 3' UTR Clone
CAT#: SC216897
3' UTR clone of deleted in azoospermia 4 (DAZ4) transcript variant 2 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | DAZ4 |
Synonyms | pDP1680; pDP1681 |
ACCN | NM_020420 |
Insert Size | 1922 bp |
Sequence Data |
>SC216897 3’UTR clone of NM_020420
The sequence shown below is from the reference sequence of NM_020420. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC CTTGTCTGTTTAATCCAGAGAAGAGACTGATAAATTCCGTTGTTACTCAAGATGACTGCTTCAAGGGTA AAAGAGTGCATCGCTTTAGAAGAAGTTTGGCAGTATTTAAATCTGTTGGATCCTCTCAGCTATCTAGTT TCATGGGAAGTTGCTGGTTTTGAATATTAAGCTAAAAGTTTTCCACTATTACAGAAATTCTGAATTTTG GTAAATCACACTGAAACTTTCTGTATAACTTGTATTATTAGACTCTCTAGTTTTATCTTAACACTGAAA CTGTTCTTCATTAGATGTTTATTTAGAACCTGGTTCTGTGTTTAATATATAGTTTAAAGTAACAAATAA TCGAGACTGAAAGAATGTTAAGATTTATCTGCAAGGATTTTTAAAAAATTGAAACTTGCATTTTAAGTG TTTAAAAGCAAATACTGACTTTCAAAAAAGTTTTTAAAACCTGATTTGAAAGCTAACAATTTTGATAGT CTGAACACAAGCATTTCACTTCTCCAAGAAGTACCTGTGAACAGTACAATATTTCAGTATTGAGCTTTG CATTTATGATTTATCTAGAAATTTACCTCAAAAGCAGAATTTTTAAAACTGCATTTTTAATCAGTGGAA CTCAATGTATAGTTAGCTTTATTGAAGTCTTATCCAAACCCAGTAAAACAGATTCTAAGCAAACAGTCC AATCAGTGAGTCATAATGTTTATTCAAAGTATTTTATCTTTTATCTAGAATCCACATATGTATGTCCAA TTTGATTGGGATAGTAGTTAGGATAACTAAAATTCTGGGCCTAATTTTTTAAAGAATCCAAGACAAACT AAACTTTACTGGGTATATAACCTTCTCAATGAGTTACCATTCTTTTTTATAAAAAAAATTGTTCCTTGA AATGCTAAACTTAATGGCTGTATGTGAAATTTGCAAAATACTGGTATTAAAGAACGCTGCAGCTTTTTT ATGTCACTCAAAGGTTAATCGGAGTATCTGAAAGGAATTGTTTTTATAAAAACATTGAAGTATTAGTTA CTTGCTATAAATAGATTTTTATTTTTGTTTTTTAGCCTGTTATATTTCCTTCTGTAAAATAAAATATGT CCAGAAGAGGCATGTTGTTTCTAGATTAGGTAGTGTCCTCATTTTATATTGTGACCACACAGCTAGAGC ACCAGAGCCCTTTTGCTATACTCACAGTCTTGTTTTCCCAGCCTCTTTTACTAGTCTTTCAGGAGGTTT GCTCTTAGAACTGGTGATGTAAAGAATGGAAGTAGCTGTATGAGCAGTTCAAAGGCCAAGCCGTGGAAT GGTAGCAATGGGATATAATACCTTTCTAAGGGAAACATTTGTATCAGTATCATTTGATCTGCCATGGAC ATGTGTTTAAAGTGGCTTTCTGGCCCTTCTTTCAATGGCTTCTTCCCTAAAACGTGGAGACTCTAAGTT AATGTCGTTACTATGGGCCATATTACTAATGCCCACTGGGGTCTATGATTTCTCAAAATTTTCATTCGG AATCCGAAGGATACAGTCTTTAAACTTTAGAATTCCCAAGAAGGCTTTATTACACCTCAGAAATTGAAA GCACCATGACTTTGTCCATTAAAAAATTATCCATAGTTTTTTTAGTGCTTTTAACATTCCGACATACAT CATTCTGTGATTAAATCTCCAGATTTCTGTAAATGATACCTACATTCTAAAGAGTTAATTCTAATTATT CCGATATGACCTTAAGGAAAAGTAAAGGAATAAATTTTTGTCTTTGTTGAAGTATTTAATAGAGTAAGG TAAAGAAGATATTAAGTCCCTTTCAAAATGGAAAATTAATTCTAAACTGAGAAAAATGTTCCTACTACC TATTGCTGATACTGTCTTTGCATAAATGAATAAAAATAAACTTTTTTTCTTCAAATGTG ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_020420.4 |
Summary | This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2011] |
Locus ID | 57135 |
MW | 74.8 |
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