Glycerol kinase (GK) (NM_001128127) Human 3' UTR Clone
CAT#: SC216797
3' UTR clone of glycerol kinase (GK) transcript variant 3 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | GK |
Synonyms | GK1; GKD |
ACCN | NM_001128127 |
Insert Size | 2000 bp |
Sequence Data |
>SC216797 3’UTR clone of NM_001128127
The sequence shown below is from the reference sequence of NM_001128127. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC GGAGCAAGGTACATCTCAGGTATTCCATAAAACCTACCAACTCATGGATTCCCAAGATGTGAGCTTTTT ACATAATGAAAGAACCCAGCAATTCTGTCTCTTAATGCAATGACACTATTCATAGACTTTGATTTTATT TATAAGCCACTTGCTGCATGACCCTCCAAGTAGACCTGTGGCTTAAAATAAAGAAAATGCAGCAAAAAG AATGCTATAGAAATATTTGGTGGTTTTTTTTTTTTTTAAACATCCACAGTTAAGGTTGGGCCAGCTACC TTTGGGGCTGACCCCCTCCATTGCCATAACATCCTGCTCCATTCCCTCTAAGATGTAGGAAGAATTCGG ATCCTTACCATTGGAATCTTCCATCGAACATACTCAAACACTTTTGGACCAGGATTTGAGTCTCTGCAT GACATATACTTGATTAAAAGGTTATTACTAACCTGTTAAAAATCAGCAGCTCTTTGCTTTTAACAGACA CCCTAAAAGTCTTCTTTTCTACATAGTTGAAGACAGCAACATCTTCACTGAATGTTTGAATAGAAACCT CTACTAAATTATTAAAATAGACATTTAGTGTTCTCACAGCTTGGATATTTTTCTGAAAAGTTATTTGCC AAAACTGAAATCCTTCAGATGTTTTCCATGGTCCCACTAATTATAATGACTTTCTGTCTGGATCTTATA GGAAAAGATACTTTCTTTTTTCTTCCATCTTTCCTTTTTATATTTTTTACTTTGTATGTATAACATACA TGCCTATATATTTTATACACTGAGGGTAGCCCATTTATAAATTAAGAGCACATTATATTCAGAAGGTTC TAACAGGGCTGGTCTTAAGTGAACCACTGTGTATATAAATATGTTGGAAAACAGCTGTATACATTTTTG GGCAACGGTTATGCATAATATTTACCAGGAGAATTTTTTTCTTAACAAGCCAACATTTAAAATTTATGT TTTATGTCAATAAAAGAAAATATACTTTATTGTGACTTCAACTATATTTCTTATCCCTTACATTTTTAT TTAATTGTCTTAGCTTAAAAAAAGAAGAAACTGTGGAATACTACAGTAAATATTGTTTTCAAACACAAG CAATAATTCAAATAGTTATTTTTCTTTTGAATTAATTTTAGACATATTTTGGATCCTATTGAGGGGATA AGAGGATGTCAAAAAAGTTAAATACCTAAGTAGAAAAAAATATAGAAATAAAGCCAAGAATCTCTTTCA GTTCAAATGTTATCAATTGTTAATAAGAAATTGCTATCTGGGATGACAGAATTACCTCTGCTTAGTATC TCATTATAACTGAAAGAAGGTTTATCATTACAAATACCTTCCAATGAAACCAAGAATTTCTCAAAATAT TTAATGTCACATATTATAAGAAGTTACCTAATCCTGCTTCTTAACATCAATTTTTAAAAATATCTTAAA ATTACTTTGTTTTGTAGTAAACAGTGAAGAAAAGATTGCCTCCTAATTATTTTTTTCAATGAGTGCTGA ATGGGAAAACATTTATATCTTACTATAAAAGGTTCTGTTTTGTTTGGAATCAATGGTAGCTTTATTGAC TGTTCTGATTGTGCTGTTTCTAATTTATTGAATCTGCTAGGTTTTATTGATGCAGCCACCACTTAAGTG ACATAAATATTATAGAAAGGTACTGTGAAATGATCACTTTGTGGCAGGGGTACTTTTAAACATAAATGT TTCTACAAAAGTAGGTTGAGTTCATTGTAAATAATTGTGAAAGCCACTGTTCAAATAATTTTAAGATTA CATTAATTTTTCTATAAATTGGAAGATTTATAAATGTTTGAAATTGTACACATTGATATTTAATGACAA ATTTACTTAAAATAAATTGACCCCTTGTTCTTACTTGCATTTCTCATTTACAGACTAGAACTTAGTTGA AAGTTAAATTAAGAAAGATGTTTCAGAGGCCGGGCACGGTGGCTGACGCCTGTAATCCCAGCACTTTG ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_001128127.3 |
Summary | The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Locus ID | 2710 |
MW | 77.1 |
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