PATL1 (NM_152716) Human 3' UTR Clone

CAT#: SC215996

3' UTR clone of protein associated with topoisomerase II homolog 1 (yeast) (PATL1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PATL1
• Synonyms: hPat1b; Pat1b
• ACCN: NM_152716
• Insert Size: 1704 bp
• Sequence Data:
  Insert Sequence

  >SC215996 3’UTR clone of NM_152716
  The sequence shown below is from the reference sequence of NM_152716. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAACTGCAGCTAGTTCAGGGGATACGATAAAAGATCTCCAAATGTGTCCTGTACCTCCTTTTGGCTGCC
  ACCTGCACTGCTGCCATCACCAATGGAGTGTTTTTAATGAGGGAAGGAAGGTAGCTTTTTCCCCAAAGC
  AAAGTCTTGTGGGATCGATTCCTGTTTACAGGGGTTGTCTCTCTAAATGTCAGATATTTCCCCACTGCT
  CTATGAAATTTGGCTGGGTGATACTTCTGCTGGTTTCTTTACCTTCTGTGTTACAGTTCTGCATGTCCT
  ACTTTTACTCAGTTCTGTTTTGCATTTTCTTTGCCCTAGAGACACAAGTGTAATCTCTCCCTTTATCCC
  TCCACTACTCCACCTCAGAGTAGATTGTAGCCTGCCAAAGGATTCCTTCCCTCATCCTATTGAAGTTGT
  TTTTTCATTGCCCCATATTAATATGACTATAGAAGAGCCAATTAAGTAGAAATCAAGATATACACACAC
  ACATAGATACACACACACACACCCCATACATGTATTTATGTGGTCTTCAGAGGGTCCTTAAAGAATGAA
  TTTTAGATTGAAAAATATTTAGTTGTCTCATTACCTCTTCTAAACACAAACCAGCTGATGTATTTTAAT
  CTGTTTCTGTTCTATCTTGTAATTAATTTGGTGGGTTCTACTTGTTTTAACATAAATAAAGAGTATGCA
  GCACGTTTAATAAAATCAGAACTCTTAATTGGCTTATGCCCAGGTCTAGGCTGAGAAGTCCTTTTTCTT
  CTTCCCACCTTTATTTCCTTAGTTTCTGTCCACCTTAATCGAAACAACACATGGTTATGTCTTTTTCCT
  GCTACAACTACAGGGTACTTGAGCCTTTCCCCTCAAGTGCATTCGAAGTCACCCAGGATGATCCTCACT
  AGTAGCCTGCTTTGGCAGTGTGGCTTTTTGCACACTTGCCCTGTCTTCCTGAGACTACTTCAGTAAGCC
  ATGCTTCCTTCTTCCCCACTTTTATTTGGTGTCATGAATAGAAACTTCCAAATGTAACCATGGAAGCTA
  AGTTTGGCCTGCTTTGCTTTTTAGTCTCCACACCATGGGCAGAACTGCTGTCTTTACTACTTCATCTCA
  CCCAAGTCCCGTTCCCAGGCAGCCAGGGGCCTGGGTTTGAATAATTGCAGGGCCAGCCTGCCATGATCT
  TTCTCACTTACTCCTCTCCCATTCAGCAATCAACCAGACTAAGGAGTTTTGATCCCTAGTGATTACAGC
  CCTGAAGAAAATTAAATCTGAATTAATTTTACATGGCCTTCGTGATCTTTCTGCTGTTCTTACTTTTTC
  GAATGTAGTTGGGGGGTGGGAGGGACAGGTTATGGTATTTAAAGAGAATAAACATTTTGCACATACATG
  TATTGTACAACAGTAAGATCCTCTGTTAAAACCAGCTGTCCTGTTCTCCATCTCCATTTCTTCCCATGC
  TGTAACCCCAGGCTCCACCAGCTGTTCCCCAGTGATGTTACCTAGCTTCCCTCTACCGTTGTCTACTGA
  CCATTTCCACTACATGCCTTTCCTACCTTCCCTTCACAACCAATCAAGTGAATACTTGATTATTATCTC
  TTCCTTACTGTGCTTTATCTTTTTTGTTTGGATTGGTTCTAATTAATGAAAATAAAAGTTTCTAAATTT
  ACATTTTTATAGGGTATTGTAAATAAAAACAAATTGTATACTTAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_152716.3
• Summary: RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV).[UniProtKB/Swiss-Prot Function]
• Locus ID: 219988
• MW: 65