AGAP1 (NM_014914) Human 3' UTR Clone

CAT#: SC214151

3' UTR clone of ArfGAP with GTPase domain ankyrin repeat and PH domain 1 (AGAP1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AGAP1
• Synonyms: AGAP-1; CENTG2; cnt-g2; GGAP1
• ACCN: NM_014914
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC214151 3’UTR clone of NM_014914
  The sequence shown below is from the reference sequence of NM_014914. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGCAGTGGGAGGGTGCCCACCATCATCTGAGGAACAGCCGTGCCCGCCTGCTCGCCGCACCTGGGACGC
  GGCAGCCTCGCCGCATTCTCGCTCAGAAGTCGCAGCACGTGAGTCCCGTCGCATCCCCTCCCTCTTCCT
  GGTGGCCACCTCCCTCCCGCCCACCCACTCTCACCCCAAACAAAATCACAAAACCTGGACATCCCTCAA
  GGGGCGAAGAGGCGGCCGGGAGACTGCAGAAGTGGCTCCTTTTCATAAACTCCCCTAAACCACACACAG
  GAGAGAGCGACGGGCCTCGGCCCTTTGATGATAGCACATGGCGCAGGACCCTTGTCCTGGTGGCACAAG
  GGATGGGGACGCGAGGGGGAGGGGAGGCGAGGAACAAGGAGAAGGGGCAACTTTCCTTAACTGGCAGTT
  GAGCACATAGTACATTTCCCCTCTACCAAACGGAACACTTGGATTCCATCTCTTCTCTGAGGAGCTCGA
  CGGCATAAATCAGAAGCAAGCACAGAGTTTGTCAGGTTTGAAGCCCCTATGATGGTGTGTGTCAAATCA
  GTTGTAGCTAATCTGTCCAGGGAGAATACTGGCTTCATTACACTTGTACAGCCGAGTTCTTCCCGCATT
  ACTGCTGTTTAATAGAACGTGATTAGTCATCGCCGAGAAGAAAGCATATTAGCCGAGGAGGTAGTCACG
  CGGCACGCGCCGGTGATTGCCACGATGTGATTGCAATACTCTTAGAAGCACCATATTATCCCAGACATG
  TTCTTTCAAGCCCTTGGAGCCCTCTCTAAATTCACTGTCATCATTTAGTATCTGTTTAATTTTTCAGTC
  CAAAGAGAGGAAATCAGTCGCTGAGTATTATTTGACTCCGGTCTCCTTGGTGCAAAAACAAAATGGGAA
  AAATAAATAAGAATAACTCAGAAACTCAAAAGGAAACCACAAATTCAGCTAATAATAGCATTTCGAGTA
  TATTTCGTAAACTAAGGAAATACACAAAAGGCTGTTTTTTCCGACTGTAAGAGATATTTGATGTCCTTT
  TGCCGAGGTGGATGTGTTAGTCTCAGGCCCTCCTGGACCACGTTGCCCAAGTCACACAGGCTTCTGTGT
  TATGTATTTAGATAAGATGTGTGAAAATATATTTGAATAAAAGAAGTTCATAAATATGCATTGATTTTT
  GTACAGACAAATGGCACCTCTCTTAATTTATAAATTGAACTGGATGTGAACTAATAATGTGCAACTAGT
  TGAGATAAGAGGGTTACAGATCATTGTACATGGAAAATATTCCCAGCAGTAAACACTTCCATTAATGTG
  ATCTACGGCTTTTGAAAAGGAGCATCTCAGAATAAGATGGTGGTACAATTTGCTTATTAAAATTGAGAA
  AGAAAAAAAAAGACACACTGGAGTATATTAGAAAGGAAAAAGAAGGAATGTGGTCTCTCATGATTGAAA
  GCATGATTTAGATCGGATACGGCTTTTGCTAACCAAGGACCAAGGGACTCTGGCAAGATGGGGTGGTTT
  TCCGAATGCCAGACCGAGGTGCCTTACGAAGGCAGCTGCCAATGGTTCTGTGGCACAGACTGGCTCCTG
  AGAACCCAGACGACCTTGACCTGGCAGCCCGGCCCTCCACGCTAGTACCTCGCCTGATTTTCCATCGTT
  GCGGTATCCAGCCGCTTCAGACGTCCGCCGCCTTCAGTGGTGGTAGTCAAATGTAGTCAACCAAACCAC
  AGCATCCCTTAGGTTAAAAGCAAACGGGGCAGCTTTGCCTAGAGCTCATTGACAGTGTGATGGGGGGAG
  AAGGGTTGTCACAATGGAAGATGAGGACACTAGACTCATGTAGCACAGAAACATGAAGCCACACGCACC
  GGCAGCCTCCGGGGTGTTCCTCGCTCTCTCGTATGTTAAACCTTATATTTTATAAGAGTTTTTCCTCTT
  ATTTCACTTTTTAAAAAAAATTAAAGTAGGTGGGGAAAAAAATAAAGCTTTACACAGAATTTATATGT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_014914.5
• Summary: This gene encodes a member of an ADP-ribosylation factor GTPase-activating protein family involved in membrane trafficking and cytoskeleton dynamics. This gene functions as a direct regulator of the adaptor-related protein complex 3 on endosomes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Locus ID: 116987
• MW: 75.1