CDC23 (NM_004661) Human 3' UTR Clone

CAT#: SC214056

3' UTR clone of cell division cycle 23 homolog (S. cerevisiae) (CDC23) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CDC23
• Synonyms: ANAPC8; APC8; CUT23
• ACCN: NM_004661
• Insert Size: 1358 bp
• Sequence Data:
  Insert Sequence

  >SC214056 3’UTR clone of NM_004661
  The sequence shown below is from the reference sequence of NM_004661. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCACTCAACTTGTCTTCTGTCACGCCATAGTTGGCTACTCTCAAGCCAGCACATTGTTAGACCCATCTT
  AATTAAGCCTTACCTCCATGTAAAGAACAGCACGTCTGTTCCAAGGACCTCAGCTCTTCTTGTTTCTAC
  AGATGGCAACAGCTCCATAGGGACAGCTTGTATAATTACCTTCAGAGGCCAACTGACAGAATCCTGGCA
  GGAACAGACATTATCTTGCCAGTTAGAAGTACTTCTGTCTCACTTATGTCCAAAGAGTGGCTATAGATC
  TTGGCCTTCTTCCCTGAATGCTTTTTTTTTTTGGCCCCCAAGAAAGTCCCTTTTATAGCACTTTAGCAC
  AGGCAATGCTACAGGAACAAAGTTTCAATGCTGCTGAGAGTGAAAGAAAGGAGGAAAGTCTGCCACTCT
  ACCCTGAGCTGGCAGTAGGGCACTGAGTACCCTAGGAAGAAGTCAGAGCAATGGATACAAATGACCTTG
  CTCTTGGATTTGCTGAGCATGATCCCTATTCTGATGTCAGAGATTAGGTTTAAATGGAATAGAGCTATC
  CATTTGTTCTTACTCTCTAGGGAGACAATCTTCCAAAACAGTTTTGGGGGGGTCTTCTAAAGCTTTCAA
  ATTGGAAGTAACTTTATTCAACTAGAGTTGAATAAAAGAAGGGCAAAAATAATCTCACAGAGCTTGGAA
  CTGCTGATAGCCCTTACTGAGGGCAAAAGATGGCTATATTGTTAGCTATACTCCTACCAAAGCAAGCAA
  GGAGATAGGATTATAGATAATTTCACGGACATTTGGAAATAACATTGGTGATTATACAGACAAGAATAA
  ACTCACTTCAAGCTGGTCTGTTTTAATAAATTTTCAACGTAATTGTCTATTTTTTTCCCTCCCATCTGC
  AACAGAATACATTTTTTTCAGCCTTTATCTAGATGAGGTAAAGGGAATCATTCTTATGGTGCTCTTGGA
  GAGTTTCAGGCCTGTGCATGTGTGTACAGCAGGAGGTAATATGCTATAATGTCTGCTGTAATATATTTG
  CACAGTAGATGCTATGGATCATTCTGAGCTCAGGGTCCAGACTTTATTCTTATTCCCAGAATTTTGTGT
  TACGTTTTTACCTCCTAACATATGACACTTCATCTTATATTAAGGAAGGTTTAGAATATCTAATACGAC
  TTGAATTCATTTGTTACTAAGCCTTCTCAGGCAAGCTGTATACTAGTTACTGGTCTCCACTGCCATGCC
  TTTTCAAGGTTCCCATGGTCCAGAATGATGTTTGATTCTTAATTTTTCTGTCCCTTTTATAATTTGTTT
  TAATGATTTTGCTACATTTGGAATTCAATAAAAAATGTGAACAATAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004661.4
• Summary: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]
• Locus ID: 8697
• MW: 53.2