TFII I (GTF2I) (NM_032999) Human 3' UTR Clone

CAT#: SC212858

3' UTR clone of general transcription factor IIi (GTF2I) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GTF2I
• Synonyms: BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6
• ACCN: NM_032999
• Insert Size: 1192 bp
• Sequence Data:
  Insert Sequence

  >SC212858 3’UTR clone of NM_032999
  The sequence shown below is from the reference sequence of NM_032999. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATCAAGCAAGAACCAGACCCCACGTGGTAGACCTCTTCCCTCCTAGGCTTAAAGTATCAGTGGTTGAGA
  AGAGCTTTTCGGACCTGTTACTACCCCAAGCTGTGTAATATACTTGTATAACAGAAATACCTTCTATAC
  AAACCTTTTTTTCTACTTTTAGATAGAAATGTCTACTTTTTCAGCAGTTCTGTGAATTAAAGAGCAGAG
  TGACTGTGGGTCTGGAATGGCTGGTGTACTTGGGAATGTACTATCAGGATTTTACAGCAATGCTGGGAA
  ATGACAGGGAAAATGACAGGAATGAATCTCACCAGATTTTTTATGTACTCAGCAGAGCCTTGAGTTACG
  GTGTTTATTTTCCAATCAAGTGAAGATATCTCCTACTTCTCCTACTGGAACATCTCAGCTTCTGCAGTG
  AAGAAAAATTCCTGTGATAGTTCAGTTCTTTAGTTTTTCTATTTGAAAAAAAAAAATCATTTAAATGAT
  CCTTTGTTCACGGCTCTCCTTAATGACTGAGTGAACAGTTCCTATCTGTATATTTGACTAAACCTTTTC
  CTAAGCTATCTCTCATGGTTCCTATGTTTTTTTATCATAATTAAAAGCAAAACCATCTGGATCACCTAA
  CAGTCAGAGGTCAGTATCTCAGCGTGTGAATTATAGAGGAAATACAGAGAGAACCTCTTCCACTTTTAC
  TTTTCGTCCAAATAAAATGCATGGTGTACCAGAAGTTGAAGATCGGGTTGAGGATTGGGGCTAGCTCGA
  TGACACTAAGGCCCCAACATCGCGGGACCTGCTGTGGCGCGGATTCTTAGGAACGCTGTTCTAGCCGGC
  CCCCTCTCCAGGGGTCGCCGTGGCCGGCATTATTTCCTAGTTCTTCTTGTAACCCTGAGGTGCCAGCGC
  GGGGAGTGAGGAGGGGTCAGGGGGCTAAGGATGCAACCTCTGACGTTCTGCGCCTTCCTAGGAGAGTCT
  TACATGTGTTGAGATTTCACAAGCAATGCGAGTTGTAAAATACCAGCTCTACAAGAAGCTAGGCTCTGT
  GACGGCATAGTTTTCAGTAGCTTTATCACAATATTCACAATGGAGAATTATATGACATGGTAGCAGAAA
  TAGGCCCTTTTATGTGTTGCTTCTATTTTACCTCAAATTGTAGATATAGGGTAATCAATAAAATCCATC
  CATGCCTTTCACACACTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_032999.4
• Summary: This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
• Locus ID: 2969
• MW: 46