AP1B1 (NM_145730) Human 3' UTR Clone

CAT#: SC212821

3' UTR clone of adaptor-related protein complex 1 beta 1 subunit (AP1B1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AP1B1
• Synonyms: ADTB1; AP105A; BAM22; CLAPB2; KIDAR
• ACCN: NM_145730
• Insert Size: 1171 bp
• Sequence Data:
  Insert Sequence

  >SC212821 3’UTR clone of NM_145730
  The sequence shown below is from the reference sequence of NM_145730. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGGCCTACGAGACCATCCTCAAGAACTGAGACCCCGGCCAGCGCCCACCCCAGCCTTCTGCCCGCCCC
  ATCGAGGAGGCCCCTCGGGGGCAGCACATCTTCCTCCTCGCAGGAGGGACCAGGCGGGGCTCCAGGCCA
  CTCAGTGGGCTCCCTGGTCCTGATGGCAGAACCCACCCGATCCCTGGGGTAGGGCACCACCCCCTCCTG
  GGGTGAGAGCGCAGTGCACTCCCGTGCTCTGGGACACCCCTGCTCCTGTGGCTGTGATGTGGGGTTAAG
  TGAGGTGGGGACCAAAGGAAACAGAGCCAGAGCAGCCACAGAAGCTGTGCCTGAAGGGTGAGTGTGGAG
  CTTGCCCCTCCGGCTCACAGCCCCGGCAGCCCCTGGCTCCTTGGTCTCTCCGGTTGGTGTTAAAGGGCC
  CTCCACTGCCACCTCTCATGGGATGGACCCTGCCAACCTGGCCTGGGTGTGCAGGGAGGGGTTCCCCTT
  GGTACCAGGAGAGCTGCTCACTTAGGGCCTGGGGCTCAAGGAGCTGTAGGTGCCGGCAGAGGGGCAGAG
  CTAGGCTGGGAGGAGCCCAGGGCCTGCACCACCCACTTGCAACCACCAGGCTGGTGCCCTGCAGCTGTG
  CCAGTTGGGCCACAGCCTCCCGAGTGCTGACCCACATGGTCAAGACCAGGCAGAAGCTCCCAGAGCCCC
  TGTCTCGGGCTCCCCACCGACTGGCAGCTGCACTATCCCCAACTCCCCACTTCTGCCCCAAGGGTGGTC
  ACTGCCTGTGATACATTCCTCAGTGTCGCCTCTGAGCCCAGGCCTCCTGCCACAAAGACTGGGCCGAGA
  GATGGGGCTGGGTAAGCGGGTGCGCCTCCTGTTTGGGTTTCTTCGGGGTTTCTCTGTAGTGTCTGCTGC
  CCTCCTCCTGGCCTCCCAGGTTTCAGTTGTGTCTGACAGAGCATTAGGTTTTCCTGTTACTGCTGTGTA
  ATAATAAAGAAAGATTCGGCTTTTGGCAATCACGGACACTTTTTTCCCCTCCACCGAATGTCAGGATTG
  AAAGCTGCCTCCGAGTGGTTGGGGATGGTTTTCTGACCCTACGGTAACTAGATCTAATATATATCATCA
  GAAAATATATATTGTATGTTTACTCTTATTTTCAGAAAGAGAAAATGAATAAAAATGATGACATCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_145730.3
• Summary: Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
• Locus ID: 162
• MW: 41.6