PCGF6 (NM_032154) Human 3' UTR Clone

CAT#: SC212627

3' UTR clone of polycomb group ring finger 6 (PCGF6) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PCGF6
• Synonyms: MBLR; RNF134
• ACCN: NM_032154
• Insert Size: 1139 bp
• Sequence Data:
  Insert Sequence

  >SC212627 3’UTR clone of NM_032154
  The sequence shown below is from the reference sequence of NM_032154. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTTGTGGTTTCTCCTCTGAAGATAACTTGAAGATTCTAGGCACATTATGAGGAGGGAAACAAAGGAGGC
  TTCTGCAGGACTGCATCTCACCAAAGATTTCCATGAAATGTAATTGCTACCACTTTGCTGTTCAAGACA
  TAACTTACCTATTTTTAGCACCAAGAATTATAGCGTTTATAAGTACAACTTGGAACGATGGAATGCATC
  TGTTACTAGAGACTGTATATAAAAAGCAGCCAAAGCTCAGGGGAAATTTTTCAAAAATTGAATTTTAAA
  ATTTCTTATGATTTTAAATACCTTGACATTGATTTTGCTTCCCATCTTTGAAGTAATTGGTTAGTTTTC
  TTTGTTCAGCCATTTCAAGTTGGTGGTTGGGATATCTGCTCTCTGGGATGGCATCAGTTGGGCAGTCAT
  CTTTTTGGAAGAGAAGTGTGCTTTTGAGTGGAAGATTCAAAGCCAGTCTTTGTTAAACTGCAGGGTGTA
  CTCTCAAAGACCAAAACTATTGGCCACTTTTGTATGTTGCCAGCATATTTTGAACTTGTACCTTTTTTT
  ATACTAGCAACACTACAAAGGAGAAGTTACTCATTTCAAAATGGATGAATTGTTATTATGTGTGAAGAG
  ACTCTGAGTTTATGATCTGTGCCATAAAATTTCAGTGTAATAAGACTTCTTCAATACATCTTCCAATAA
  GGGGTGCTTCTTTGTGACAGTATTTTTATTTCTGACATTCATTTTATTTGGGTACATAGTGTGGTTGTT
  GATACCTTGCAATAGTATTGCTTCTGAAAGTAATAAAAAATTTTAGGAGAATTTGAGAAGTTTACAGAA
  TTACTTATTCATTGTTTTCTTAGTAAGTCAGTTTAATGTTTATTTTTCTCATTATTTCATCACTGCAAT
  AAAGAATAAGGGTGTTTGAGCTCACCTCCATGCAAAGACTTCAGTTTTAAAACATTATTTGCCTAAAAT
  TAGCATTGTGATGCTTTCTGAAAGAAATATTTTATAAATCCTGTTTAACAGCAGAATTTCTGAATGTGT
  AGAGACTACTACAAAATTGGAAAAAGATTGGCAATTCTTTGTAAAGAATTTGTGAATTGTGTATAAATT
  TATTCTAACATTTAATAAAAATGTATTTTAACCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_032154.4
• Summary: The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
• Locus ID: 84108
• MW: 44.1