GEF H1 (ARHGEF2) (NM_001162384) Human 3' UTR Clone

CAT#: SC212535

3' UTR clone of Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ARHGEF2
• Synonyms: GEF; GEF-H1; GEFH1; Lfc; LFP40; NEDMHM; P40
• ACCN: NM_001162384
• Insert Size: 1118 bp
• Sequence Data:
  Insert Sequence

  >SC212535 3’UTR clone of NM_001162384
  The sequence shown below is from the reference sequence of NM_001162384. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GACGGGGAGGCTGTAGCCTCCGAGAGCTAAGGGGGCCCCTCCCCCCTGCCCCGTGCCCCACTGAAGAAC
  ATTACTGAGGGGGCTAACCTTGGGGACTCCAATTTGCCAATGATGAGGGAACATTTGAAAGAACTGCAA
  ATTGTCCTTGCCAGCTCTTGGGATCCTTGGATACCTGGGGCCATTTAAGAAGCTAGGGGAATTAGGCCA
  CAACACCCCCTGGGACATCCGAAAGCTACACCACAGATGCCAGTGGTTCATGCCTTCTTCCCGCAACTT
  TAGGAAAATTTATTTATTTATTGTTTATTAGTTATGGGGGGAGAGGGGAGATTTAAAGGACCAGGGACA
  TGGGAACCAAGCCATAGGGATCAGAGGGCCTTGTCCTTGAACACTACTGGGGTATATTCAGGCTCATCC
  ACGCAGCTGCTGGGTTCTTGCCCTAACGGCCCTCCCCTGCAACATCCGTCTTGGAGGAGAGGCTGCAGC
  CACAGCACCCTACTGCCCTTTAAATAAAGGAGGGCTGTGGGCAGGGCCATGTCCCTTTCTCCTCTCCCC
  TCAACCTCTTACTGCTGTTCTCCCTTTCTCCGTCCTTCATGGAAGCCCTGGGAGATAACCTGGCTTCCT
  GGAGTTGATGGAATAAAGGTTGGGGTGGCCATAATGGTTTGTTGGGGGTGAGGGAAAAAACCCACAGGG
  ACCAGAATGTTTTGTTGTTCTTTTGTTTTCTTTTTTGTACCAAAGTCAACTGCACGTGTTTTATATTTT
  TAAGAGATCGTAGGCAATTAGAGATCGAAGCCTCCTATCTCCACATCTCTGAAGAAGTTGAGGGGTGGG
  GGAGAGAATGACTTCTGCCTTCATCTGCAGTAACGGGGGGACCTATACTGACCTCTTCCCCAGCCATTT
  AGAAACAAGTTCTAGGGTGGGTTGGAAAATCTCCAAGAGCCCTGACCTCATCTTCCACCTCAGCAACCA
  TGACCTGAAACCTCAGCGTGAATTTGGGGGATTTTTCAGTGGAACCCTTGCCCCCAAATGTCGACCAGC
  CCCCAAATGTCGAAGAATTTTCTTCTTGCCAATTTTGTTGTTTAAAAAAAAAATTCAGGGAAAATTAAA
  AACCTGGAACTCCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001162384.2
• Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
• Locus ID: 9181
• MW: 41.2