SIAH1 (NM_003031) Human 3' UTR Clone

CAT#: SC211989

3' UTR clone of seven in absentia homolog 1 (Drosophila) (SIAH1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SIAH1
• Synonyms: BURHAS; SIAH1A
• ACCN: NM_003031
• Insert Size: 1079 bp
• Sequence Data:
  Insert Sequence

  >SC211989 3’UTR clone of NM_003031
  The sequence shown below is from the reference sequence of NM_003031. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGCATCAATGTAACTATTTCCATGTGTTGAAATGGCAATCAAACATTTTCTGGCCAGTGTTTAAAACTT
  CAGTTTCACAGAAAATAAGGCACCCATCTGTCTGCCAACCTAAAACTCTTTCGGTAGGTGGAAGCTAGA
  CACATGAAGGTAAATAAAAAGAAAGGCTGTTAAATACAGGAAACAGTTGCATGTAGTAACACTAATATA
  TTTAAAAATAAGTCAACAGTAAACCACTGAAAAAATATATGTATATACACCCAAGATGGGCATCTTTTG
  TATTAAGAAAGGAAGCATTGTAAAATAATTCTGAGTTTTGTGTTTGTTGTAGATTGATTGTATTGTTGA
  AAAAGTTTGTTTTTGCGTGGGAGTGTGTGCCTGCGTGGGTGTGTGCGTGTTTGGGTTTTTTTCCTTTAA
  CTGACAAGCCATCTTGAGTGGTCATGGGCCACTGCTTTTCCCTTTGTGAGTCAATACATAGTGCTGCTG
  TGTGCTTTTTTTGTGTGTATTTGCTAATTTTTATTAATTTTAGTTTTTCATTAAATAAATTTGACTTTT
  CTGTAATTCAGGTTTTTCCTTTTTTTGTACCATTTTAAAGTTAGTATCTTTTGATATGCATATTTGTTT
  ATGGTAAAAAATTTATAACGTGTTCAATATTTTCTTTTCCCCCATTAATCAGTTCATTAGAAATATTTT
  AAAATCAGCTATTTTGTGAAGCCATGAGTTCCAGAAAGTAAAGGTGACATCGGAAAAATAATCAAAAGC
  TATTTAAAGCATCTATAAGGTGCTCTCTTTCTGTCTTCTACAGATGAGTCACACCTTTGAGCTTAATCT
  TTGAAAGGTTAGAGAATAAATTGATTTTTATAAATACTGCAAATCAGGCTTTTGTTTCCTTTTTCAGAT
  ATCTTGGACAAATCACATATTTTAAAATTTGTTCTTGTATTTATTGGTTTTGCAGAAGAAGGCATCGTC
  ATGCACAGTATTTGTAATTAAAAGCAAATCATTTGTTTAAAAAGGCAGTTTGCAAAAAATGTTTTTGGT
  CTTTTATAATTCTCATTAAAAGAATATCTGGCAAATTAAAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003031.4
• Summary: This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
• Locus ID: 6477
• MW: 41.9