Nogo A (RTN4) (NM_207521) Human 3' UTR Clone

CAT#: SC211651

3' UTR clone of reticulon 4 (RTN4) transcript variant 5 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RTN4
• Synonyms: ASY; Nbla00271; Nbla10545; NI220/250; NOGO; NSP; NSP-CL; RTN-X; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C
• ACCN: NM_207521
• Insert Size: 997 bp
• Sequence Data:
  Insert Sequence

  >SC211651 3’UTR clone of NM_207521
  The sequence shown below is from the reference sequence of NM_207521. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATCCCTGGATTGAAGCGCAAAGCTGAATGAAAACGCCCAAAATAATTAGTAGGAGTTCATCTTTAAAGG
  GGATATTCATTTGATTATACGGGGGAGGGTCAGGGAAGAACGAACCTTGACGTTGCAGTGCAGTTTCAC
  AGATCGTTGTTAGATCTTTATTTTTAGCCATGCACTGTTGTGAGGAAAAATTACCTGTCTTGACTGCCA
  TGTGTTCATCATCTTAAGTATTGTAAGCTGCTATGTATGGATTTAAACCGTAATCATATCTTTTTCCTA
  TCTATCTGAGGCACTGGTGGAATAAAAAACCTGTATATTTTACTTTGTTGCAGATAGTCTTGCCGCATC
  TTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAAAAAAGCCCTTTTCAGTTTGTGCACTGTG
  TATGGTCCGTGTAGATTGATGCAGATTTTCTGAAATGAAATGTTTGTTTAGACGAGATCATACCGGTAA
  AGCAGGAATGACAAAGCTTGCTTTTCTGGTATGTTCTAGGTGTATTGTGACTTTTACTGTTATATTAAT
  TGCCAATATAAGTAAATATAGATTATATATGTATAGTGTTTCACAAAGCTTAGACCTTTACCTTCCAGC
  CACCCCACAGTGCTTGATATTTCAGAGTCAGTCATTGGTTATACATGTGTAGTTCCAAAGCACATAAGC
  TAGAAGAAGAAATATTTCTAGGAGCACTACCATCTGTTTTCAACATGAAATGCCACACACATAGAACTC
  CAACATCAATTTCATTGCACAGACTGACTGTAGTTAATTTTGTCACAGAATCTATGGACTGAATCTAAT
  GCTTCCAAAAATGTTGTTTGTTTGCAAATATCAAACATTGTTATGCAAGAAATTATTAATTACAAAATG
  AAGATTTATACCATTGTGGTTTAAGCTGTACTGAACTAAATCTGTGGAATGCATTGTGAACTGTAAAAG
  CAAAGTATCAATAAAGCTTATAGACTTAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_207521.2
• Summary: This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
• Locus ID: 57142
• MW: 38.5