ABHD6 (NM_020676) Human 3' UTR Clone

CAT#: SC211604

3' UTR clone of abhydrolase domain containing 6 (ABHD6) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ABHD6
• ACCN: NM_020676
• Insert Size: 999 bp
• Sequence Data:
  Insert Sequence

  >SC211604 3’UTR clone of NM_020676
  The sequence shown below is from the reference sequence of NM_020676. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AACACAGACAACAACAAGAAGCTGGACTGAGGCCCCGACTGCAGCCTGCATTCTGCACACAGCATCTGC
  TCCCATCCCCCAAGTCTGACGCAGCCACCACTCTCAGGGATCCTGCCCCAAATGCGGTCGGAGCGCCAG
  TGACCCTGAGGAAGCCCGTCCCTTATCCCTGGTATCCACGGTTCCCCAGAGCTTTGGGGACCACGCGAA
  AACCTCCAAGATATTTTTCACAAAATAGAAACTCATATGGAACAAAATAAGAAACCCCAGCCATGAAAT
  CTACCATGAAGTCTTCAAGTTCATGTCACTGAGAAGCTTGTGCAAAGCAGCCACCTTGGACCATAATTA
  AATCAAGGACATTTTCTTTGAGACATTCCTTATAGTTGGAGACTCAAGATATTTTTGTTGCATCAGGTG
  TATTCCCTTGCATGGGCAGTGGCTTTTATAGGAGCATTAGTCCTCATTCGCTGAACCCTGTTGTTTAGG
  TCTAATTTAAGTTTTACATAGAGACCCATGTATGACTGCAGCCCATTGGCTGCAAGACCAGGGAGGAAA
  GTGGCAAGCTGTAGAAAATGTTTACACGCATGGAGGGGCATTGCTCTAGCCCTCAGAGCGTCCGGAGCA
  GCAGGGTACATGGGTGGGAGGTTCATTCAGCACCCACCAGTCAGGTATGTTCTGAGTGAACCCACAGCA
  GTCGCAGAATGAGCACCTGGCAGGGTGGGTTTCCTAGGAATAATTTATTATTTTTAAAAATAGGCCTAA
  TAAAGCAATAATGTTCTAGACATCTGTCTAAGTAATCAGACTCAGGTTCCACACACAAGCAACAACTCG
  TGGGCCTCTTTTCTATTTCAATGTGCTACTAAGAACCCTTGGATGTAACATACTAGTTAGTTAATGAAT
  TCTGTGAATTCTGTGAAGAGTAATGTGATTGAAAATAAGTCTAAACAGCTGTAAAAGTGACCACAATGA
  CATGAAATAAATTTAATAAGTCTAGATCAGCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_020676.7
• Summary: Lipase that preferentially hydrolysis medium-chain saturated monoacylglycerols including 2-arachidonoylglycerol (PubMed:22969151). Through 2-arachidonoylglycerol degradation may regulate endocannabinoid signaling pathways (By similarity). Also has a lysophosphatidyl lipase activity with a preference for lysophosphatidylglycerol among other lysophospholipids (By similarity). Also able to degrade bis(monoacylglycero)phosphate (BMP) and constitutes the major enzyme for BMP catabolism (PubMed:26491015). BMP, also known as lysobisphosphatidic acid, is enriched in late endosomes and lysosomes and plays a key role in the formation of intraluminal vesicles and in lipid sorting (PubMed:26491015).[UniProtKB/Swiss-Prot Function]
• Locus ID: 57406
• MW: 37.2