KCNK1 (NM_002245) Human 3' UTR Clone

CAT#: SC211552

3' UTR clone of potassium channel subfamily K member 1 (KCNK1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNK1
• Synonyms: DPK; HOHO; K2P1; K2p1.1; KCNO1; TWIK-1; TWIK1
• ACCN: NM_002245
• Insert Size: 1014 bp
• Sequence Data:
  Insert Sequence

  >SC211552 3’UTR clone of NM_002245
  The sequence shown below is from the reference sequence of NM_002245. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCCTGCGTGGATGGCCCTGCAAACCATTGAGCGTAGGATTTGTTGCATTATGCTAGAGCACCAGGGTCA
  GGGTGCAAGGAAGAGGCTTAAGTATGTTCATTTTTATCAGAATGCAAAAGCGAAAATTATGTCACTTTA
  AGAAATAGCTACTGTTTGCAATGTCTTATTAAAAAACAACAAAAAAAGACAAATGGAACAAAGAAGCTG
  TGACCCCAGCAGGATGTCTAATATGTGAGGAAATGAGATGTCCACCTAAAATTCATATGTGACAAAATT
  ATCTCGACCTTACATAGGAGGAGAATACTTGAAGCAGTATGCTGCTGTGGTTAGAAGCAGATTTTATAC
  TTTTAACTGGAAACTTTGGGGTTTGCATTTAGATCATTTAGCTGATGGCTAAATAGCAAAATTTATATT
  TAGAAGCAAAAAAAAAAAGCATAGAGATGTGTTTTATAAATAGGTTTATGTGTACTGGTTTGCATGTAC
  CCACCCAAAATGATTATTTTTGTAGAATCTAAGTTAAACTTACTATTTATAATGCATAGGTAACCATTA
  ACTATGTACATATAAAGTATAAATATGTTTATATTCTGTACATATGGTTTAGGTCACCAGATCCTAGTG
  TAGTTCTGAAACTAAGACTATAGATATTTTGTTTCTTTTGATTTCTCTTTATACTAAAGAATCCAGAGT
  TGCTACAATAAAATAAGGGGAATAATAAACTTGAGAGTGAATAACCATAGTATTCTGCTGCAATAAATG
  TTTCTACCCTCTTGCGATAGAATGGTGTCAGATGTCTAACATGTTTATAAACATTTTTTTGAATTGAAC
  AGGTTATGAAAAGGATTTATTAAAAGGTTAAGATACTTTGTTTTGAAAGTACATTGTGATTTGCAGCCA
  CCTAGCACTAAAGCATAGAACTTAAACACGAATCCCTAAATGAACAGATTATGTTACATTAATCTGAGA
  ATTTTAAATTTAGAAGCTAAAATAAATAAAAATCACAGCTGGTTGTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_002245.4
• Summary: This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008]
• Locus ID: 3775
• MW: 39.6