Endothelin B Receptor (EDNRB) (NM_003991) Human 3' UTR Clone

CAT#: SC211492

3' UTR clone of endothelin receptor type B (EDNRB) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EDNRB
• Synonyms: ABCDS; ET-B; ET-BR; ETB; ETB1; ETBR; ETRB; HSCR; HSCR2; WS4A
• ACCN: NM_003991
• Insert Size: 985 bp
• Sequence Data:
  Insert Sequence

  >SC211492 3’UTR clone of NM_003991
  The sequence shown below is from the reference sequence of NM_003991. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TGGCCAGAAAGAAAGAGCAATAATAATTAATTCACACACCATATGGATTCTATTTATAAATCACCCACA
  AACTTGTTCTTTAATTTCATCCCAATCACTTTTTCAGAGGCCTGTTATCATAGAAGTCATTTTAGACTC
  TCAATTTTAAATTAATTTTGAATCACTAATATTTTCACAGTTTATTAATATATTTAATTTCTATTTAAA
  TTTTAGATTATTTTTATTACCATGTACTGAATTTTTACATCCTGATACCTTTTCCTTCTCCATGTCAGT
  ATCATGTTCTCTAATTATCTTGCCAAATTTTGAAACTACACACAAAAAGCATACTTGCATTATTTATAA
  TAAAATTGCATTCAGTGGCTTTTTAAAAAAATGTTTGATTCAAAACTTTAACATACTGATAAGTAAGAA
  ACAATTATAATTTCTTTACATACTCAAAACCAAGATAGAAAAAGGTGCTATCGTTCAACTTCAAAACAT
  GTTTCCTAGTATTAAGGACTTTAATATAGCAACAGACAAAATTATTGTTAACATGGATGTTACAGCTCA
  AAAGATTTATAAAAGATTTTAACCTATTTTCTCCCTTATTATCCACTGCTAATGTGGATGTATGTTCAA
  ACACCTTTTAGTATTGATAGCTTACATATGGCCAAAGGAATACAGTTTATAGCAAAACATGGGTATGCT
  GTAGCTAACTTTATAAAAGTGTAATATAACAATGTAAAAAATTATATATCTGGGAGGATTTTTTGGTTG
  CCTAAAGTGGCTATAGTTACTGATTTTTTATTATGTAAGCAAAACCAATAAAAATTTAAGTTTTTTTAA
  CAACTACCTTATTTTTCACTGTACAGACACTAATTCATTAAATACTAATTGATTGTTTAAAAGAAATAT
  AAATGTGACAAGTGGACATTATTTATGTTAAATATACAATTATCAAGCAAGTATGAAGTTATTCAATTA
  AAATGCCACATTTCTGGTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003991.4
• Summary: The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
• Locus ID: 1910
• MW: 39.7