DHCR7 (NM_001163817) Human 3' UTR Clone

CAT#: SC211397

3' UTR clone of 7-dehydrocholesterol reductase (DHCR7) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DHCR7
• Synonyms: SLOS
• ACCN: NM_001163817
• Insert Size: 994 bp
• Sequence Data:
  Insert Sequence

  >SC211397 3’UTR clone of NM_001163817
  The sequence shown below is from the reference sequence of NM_001163817. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCTTACCGCCTGCTGCCTGGAATCTTCTAAGGGCACGCCCTAGGGAGAAGCCCTGTGGGGCTGTCAAGA
  GCGTGTTCTGCCAGGTCCATGGGGGCTGGCATCCCAGCTCCAACTCGAGGAGCCTCAGTTTCCTCATCT
  GTAAACTGGAGAGAGCCCAGCACTTGGCAGGTGTCCAGTACCTAATCACGCTCTGTTCCTTGCTTTTGC
  CTTCAAGGGAATTCCGAGTGTCCAGCACTGCCGTATTGCCAGCACAGACGGATTTTCTCTAATCAGTGT
  CCCTGGGGCAGGAGGATGACCCAGTCACCTTTACTAGTCCTTTGGAGACAATTTACCTGTATTAGGAGC
  CCAGGCCACGCTACACTCTGCCCACACTGGTGAGCAGGAGGTCTTCCCACGCCCTGTCATTAGGCTGCA
  TTTACTCTTGCTAAATAAAAGTGGGAGTGGGGCGTGCGCGTTATCCATGTATTGCCTTTCAGCTCTAGA
  TCCCCCTCCCCTGCCTGCTCTGCAGTCGTGGGTGGGGCCCGTGCGCCGTTTCTCCTTGGTAGCGTGCAC
  GGTGTTGAACTGGGACACTGGGGAGAAAGGGGCTTTCATGTCGTTTCCTTCCTGCTCCTGCTGCACAGC
  TGCCAGGAGTGCTCTGCCTGGAGTCTGCAGACCTCAGAGAGGTCCCAGCACCGGCTGTGGCCTTTCAGG
  TGTAGGCAGGTGGGCTCTGCTTCCCGATTCCCTGTGAGCGCCCACCCTCTCGAAAGAATTTTCTGCTTG
  CCCTATGACTGTGCAGACTCTGGCTCGAGCAACCCGGGGAACTTCACCCTCAGGGGCCTCCCACACCTT
  CTCCAGCGAGGAGGTCTCAGTCCCAGCCTCGGGAGGGCACCTCCTTTTCTGTGCTTTCTTCCCTGAGGC
  ATTCTTCCTCATCCCTAGGGTGTTGTGTAGAACTCTTTTTAAACTCTATGCTCCGAGTAGAGTTCATCT
  TTATATTAAACTTCCCCTGTTCAAATAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001163817.2
• Summary: This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]
• Locus ID: 1717
• MW: 36.1