Carboxypeptidase H (CPE) (NM_001873) Human 3' UTR Clone

CAT#: SC209550

3' UTR clone of carboxypeptidase E (CPE) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: Carboxypeptidase H
• Synonyms: CPH; IDDHH
• ACCN: NM_001873
• Insert Size: 967 bp
• Sequence Data:
  Insert Sequence

  >SC209550 3’UTR clone of NM_001873
  The sequence shown below is from the reference sequence of NM_001873. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAAATGATGTCAGAAACTTTAAATTTTTAAAAAGGCTTCTAGTTAGCTGCTTTAAATCTATCTATATAA
  TGTAGTATGATGTAATGTGGTCTTTTTTTTAGATTTTGTGCAGTTAATACTTAACATTGATTTATTTTT
  TAATCATTTAAATATTAATCAACTTTCCTTAAAATAAATAGCCTCTTAGGTAAAAATATAAGAACTTGA
  TATATTTCATTCTCTTATATAGTATTCATTTTCCTACCTATATTACACAAAAAAGTATAGAAAAGATTT
  AAGTAATTTTGCCATCCTAGGCTTAAATGCAATATTCCTGGTATTATTTACAATGCAGAATTTTTTGAG
  TAATTCTAGCTTTCAAAAATTAGTGAAGTTCTTTTACTGTAATTGGTGACAATGTCACATAATGAATGC
  TATTGAAAAGGTTAACAGATACAGCTCGGAGTTGTGAGCACTCTACTGCAAGACTTAAATAGTTCAGTA
  TAAATTGTCGTTTTTTTCTTGTGCTGACTAACTATAAGCATGATCTTGTTAATGCATTTTTGATGGGAA
  GAAAAGGTACATGTTTACAAAGAGGTTTTATGAAAAGAATAAAAATTGACTTCTTGCTTGTACATATAG
  GAGCAATACTATTATATTATGTAGTCCGTTAACACTACTTAAAAGTTTAGGGTTTTCTCTTGGTTGTAG
  AGTGGCCCAGAATTGCATTCTGAATGAATAAAGGTTAAAAAAAAATCCCCAGTGCATGTATTTCAGTTC
  TCTAAGATTTTTGGTCTGGCATTGTGGTCTTATGATGAAAACATGTATCCTCTTAATGGCGGCTTCTCA
  TATATTCATCCTGTACATTTTAAAATTTGTTAAGCAACTACTGTGTGCTAAGCACTGCATAGCCTCTAT
  GTATGTTATTGTGGCAGGCAAAAGGTTCTGATTTTGGGCACAACCTTTATATAAATAACACAAAAATTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001873.4
• Summary: This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015]
• Locus ID: 1363
• MW: 37.6