SPG7 (NM_003119) Human 3' UTR Clone

CAT#: SC208906

3' UTR clone of spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SPG7
• Synonyms: CAR; CMAR; PGN; SPG5C
• ACCN: NM_003119
• Insert Size: 703 bp
• Sequence Data:
  Insert Sequence

  >SC208906 3’UTR clone of NM_003119
  The sequence shown below is from the reference sequence of NM_003119. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGAGGCGAAGAGCCGACTTGGCCCAAGTAGTTGGGAGGTGTTGGCTGCACGTGCGGGTGGTCCGGGAAG
  TGAGGGCTCACTCAGCCACCCTGAGTTGCTTTTCAGCTGAGGTTTGCACTTCCTCTCGCGGCCCTCAGT
  AGTCCCTGCACAGTGACTTCTGAGATCTGTTGATTGATGACCCTTTTCATGATTTTAAGTTTCTCTGCA
  GAAACTACTGACGGAGTCCTGTGTTTGTGAGTCGTTTCCCCTATGGGGAAGGTTATCAGTGCTTCCCGA
  GTGAGCATGGAACACTTCGAGTTCCCAGGGTTATAGACAGTCGTTCCCAGTGTGGCTGAGGCCACCCAG
  AGGCAGCAGAGCATTCAGACTCCAAACAGACCCCTGTTCATGCCGACGCTTGCACGACCGCCCCAGTTC
  CTGTGGCTCCCTCGGAATGCTAAGGGGATCGGACATGAAAGGACCCTGTGAGCCGATTGTCCTATCTCC
  AGCGGCCCTGTCATCCAGCTCACTCATCAATGGGGCCAGTCAGGCCCAGGCACTGGGCTCCGGAGGACT
  CACCACTGCCCCCTGCTGCCATGTGGACTGGTGCAAGTTGAGGACTTCTTGCTGGTCTAGTCACGCATG
  CAGTGTTGGGGATGCCTTGGTTTTTACTGCTCTGAGAATTGTTGAGATACTTTACTAATAAACTGTGTA
  GTTGGAAAATCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_003119.4
• Summary: This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014]
• Locus ID: 6687
• MW: 25.3