WNT10A (NM_025216) Human 3' UTR Clone

CAT#: SC208569

3' UTR clone of wingless-type MMTV integration site family member 10A (WNT10A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: WNT10A
• Synonyms: OODD; SSPS; STHAG4
• ACCN: NM_025216
• Insert Size: 687 bp
• Sequence Data:
  Insert Sequence

  >SC208569 3’UTR clone of NM_025216
  The sequence shown below is from the reference sequence of NM_025216. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATCACCGAGTGGGTCAGCGTCTGCAAGTGAGCGGCCCGGGGTCCCCTGGGCCCTGATCGAGGTCCCCTC
  CTGGAGCCTGGCCCTCTGAGGCTTACGGTCTTGGCAAGGCAGCATCGCCTTGGCTCTTGGGAAGAGGAG
  ATTGGACCACATGATCTTATAGGAACCCCTCAGCTCTGAGGTCTGTGATCGCCGGACAGTCCAGGCCTG
  TCTGAACCCCACCACTCACTTCTGTGGGCTCTAGGACTGACTGGGTTCTTCCTCCCTCCCCGAAGCCCA
  GACAGTTCAGTTGGGCTGGGGGTTGCTCCACACCCTAAAACAAGCCTCAGCCAGGCAACCCGTCAGTCT
  GTCTCCATCCTTTCACCCCTTCCCTGGAGATGGGAGGTGGGGAATGAATGGAAGCTGACGGGCAGAGAG
  AGGAGGATTAAAAAAAAGAAATAGACATAACTGAGCTGAAGTAATTCCATAAAGGGCCCAGACAGCCTC
  CTCCACCATTCCCTTCATCATTCATTTAACAAATATTTATTTTGCACTCTCTTTGCGGCACTCTGGGGG
  CGGTGGGGTGCGTGGGGGTGGCAATGCAAGGCACTGAGGCCACAGATGTGAGTAAGCGAGACACAACAC
  TTGTCCTCTTGGAGGTTACATTCTTGCTGGGGGGAGGCATGGGCAATAAACAAGTAAATATACAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_025216.3
• Summary: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq, Jul 2008]
• Locus ID: 80326
• MW: 25.7