NT5C3 (NT5C3A) (NM_001002010) Human 3' UTR Clone

CAT#: SC207947

3' UTR clone of 5'-nucleotidase cytosolic III (NT5C3) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NT5C3
• Synonyms: cN-III; hUMP1; NT5C3; P5'N-1; P5N-1; p36; PN-I; POMP; PSN1; UMPH; UMPH1
• ACCN: NM_001002010
• Insert Size: 630 bp
• Sequence Data:
  Insert Sequence

  >SC207947 3’UTR clone of NM_001002010
  The sequence shown below is from the reference sequence of NM_001002010. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCCAACTCTATTTTACAGAAGATTCTATAAACAAGCATTCTCCAAGAAGACCTCTCTCCTGTGGGTGCA
  ATTGAACTGTTCATCCGTTCATCTTGCTGAGAGACTTATTTATAATATATCCTTACTCTCGAAGTGTTC
  CCTTTGTATAACTGAAGTATTTTCAGATATGGTGAATGCATTGACTGGAAGCTCCTTTTCTCCACCTCT
  CTCAACACACTCCTCACCGTATCTTTTAACCCATTTAAAAAAAAAAAAAAGCTAAAATTAGAAAAATAA
  CTCCCTACTTTTCCAAAGTGAATTTTGTAGTTTAATGTTATCATGCAGCTTTTGAGGAGTCTTTTACAC
  TGGGAAAGTTTGTAGAAATTTTAAAATAAGTTTTATGAAATGGTGAAATAATATGCATGATTTTAAGTA
  TTGCCATTTTTGTAATTTGGGTTATTATGCTGATGGTATCACCATCTCTTGAAATTGTGTTAGGTTTGG
  TTATTTTGTCTGGGGAAAAAATATTTACTGGAAAAGACTAGCAGTTAGTGTTGGAAAAACCTGGTGGTG
  TTTACAATGTTGCTAATCATTACAAAACATTCTATATTGAAGCACTGATAATAAATATGAAATGCAAAA
  CCTTTTTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001002010.5
• Summary: This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012]
• Locus ID: 51251
• MW: 24.7