SMAD6 (NM_005585) Human 3' UTR Clone

CAT#: SC206393

3' UTR clone of SMAD family member 6 (SMAD6) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SMAD6
• Synonyms: AOVD2; HsT17432; MADH6; MADH7
• ACCN: NM_005585
• Insert Size: 1344 bp
• Sequence Data:
  Insert Sequence

  >SC206393 3’UTR clone of NM_005585
  The sequence shown below is from the reference sequence of NM_005585. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTGGAGATCCTCCTCAACAACCCCAGATAGTGGCGGCCCCGGCGGGAGGGGCGGGTGGGAGGCCGCGGC
  CACCGCCACCTGCCGGCCTCGAGAGGGGCCGATGCCCAGAGACACAGCCCCCACGGACAAAACCCCCCA
  GATATCATCTACCTAGATTTAATATAAAGTTTTATATATTATATGGAAATATATATTATACTTGTAATT
  ATGGAGTCATTTTTACAATGTAATTATTTATGTATGGTGCAATGTGTGTATATGGACAAAACAAGAAAG
  ACGCACTTTGGCTTATAATTCTTTCAATACAGATATATTTTCTTTCTCTTCCTCCTTCCTCTTCCTTAC
  TTTTTATATATATATATAAAGAAAATGATACAGCAGAGCTAGGTGGAAAAGCCTGGGTTTGGTGTATGG
  TTTTTGAGATATTAATGCCCAGACAAAAAGCTAATACCAGTCACTCGATAATAAAGTATTCGCATTATA
  GTTTTTTTTAAACTGTCTTCTTTTTACAAAGAGGGGCAGGTAGGGCTTCAGCGGATTTCTGACCCATCA
  TGTACCTTGAAACTTGACCTCAGTTTTCAAGTTTTACTTTTATTGGATAAAGACAGAACAAATTGAAAA
  GGGAGGAAAGTCACATTTACTCTTAAGTAAACCAGAGAAAGTTCTGTTGTTCCTTCCTGCCCATGGCTA
  TGGGGTGTCCAGTGGATAGGGATGGCGGTGGGGAAAAGAATACACTGGCCATTTATCCTGGACAAGCTC
  TTCCAGTCTGATGGAGGAGGTTCATGCCCTAGCCTAGAAAGGCCCAGGTCCATGCCCCCCATCTTTGAG
  TTATGAGCAAGCTAAAAGAAGACACTATTTCTCACCATTTTGTGGAAATGGCCTGGGGAACAAAGACTG
  AAATGGGCCTTGAGCCCACCTGCTACCTTGCAGAGAACCATCTCGAGCCCCGTAGATCTTTTTAGGACC
  TCCACAGGCTATTTCCCACCCCCCAGCCAAAAATAGCTCAGAATCTGCCCATCCAGGGCTGTATTAATG
  ATTTATGTAAAGGCAGATGGTTTATTTCTACTTTGTGAAAGGGAAAAGTTGAGGTTCTGGAAGGTTAAA
  TGATTTGCTCATGAGACAAAATCAAGGTTAGAAGTTACATGGAATTGTAGGACCAGAGCCATATCATTA
  GATCAGCTTTCTGAAGAATATTCTCAAAAAAAGAAAGTCTCCTTGGCCAGATAACTAAGAGGAATGTTT
  CATTGTATATCTTTTTTCTTGGAGATTTATATTAACATATTAAGTGCTCTGAGAAGTCCTGTGTATTAT
  CTCTTGCTGCATAATAAATTATCCCCAAACTTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005585.5
• Summary: The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]
• Locus ID: 4091
• MW: 52.6