KCNQ2 (NM_172108) Human 3' UTR Clone

CAT#: SC206140

3' UTR clone of potassium voltage-gated channel KQT-like subfamily member 2 (KCNQ2) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNQ2
• Synonyms: BFNC; DEE7; EBN; EBN1; ENB1; HNSPC; KCNA11; KV7.2
• ACCN: NM_172108
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC206140 3’UTR clone of NM_172108
  The sequence shown below is from the reference sequence of NM_172108. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GACGTGGGCTGGGCCGGGCCCAGGAAGTGAGGCGGCGCTGGGCCAGTGGACCCGCCCGCGGCCCTCCTC
  AGCACGGTGCCTCCGAGGTTTTGAGGCGGGAACCCTCTGGGGCCCTTTTCTTACAGTAACTGAGTGTGG
  CGGGAAGGGTGGGCCCTGGAGGGGCCCATGTGGGCTGAAGGATGGGGGCTCCTGGCAGTGACCTTTTAC
  AAAAGTTATTTTCCAACAGGGGCTGGAGGGCTGGGCAGGGCCCTGTGGCTCCAGGAGCAGCGTGCAGGA
  GCAAGGCTGCCCTGTCCACTCTGCTCAGGGCCGCGGCCGACATCAGCCCGGTGTGAGGAGGGGCGGGAG
  TGATGACGGGGTGTTGCCAGCGTGGCAACAGGCGGGGGGTTGTCTCAGCCGAGCCCAGGGGAGGCACAA
  AGGGCAGGCCTGTTCCCTGAGGACCTGCGCAAAGGGCGGGCCTGTTTGGTGAGGACCTGCGGCCTTGGG
  TCCCGGTGGGGTTTCCGGGCAGCTACAGGCGGGTGTGGCCGGCCGCTGTGCGTGGCCTCTGCCTTCACA
  CCTGACCTGCCCGGCGGGCTTTCCTGTTCCCCACCTCAGGGGCGCCCAAATACAGAGCTATTGGTTGGC
  GTCTTCTCCCTGTACCTTCTGGGATCTGAGGGCTCTTTCCATGGAAGCCAGCCCCGAGGTGGAGACCTT
  CGCCTGCAGCCGAGGAGCGGGTGGGGCCTGGGAACCAAACTGGAGCCAGAGTGGACGTCCAGCCCTCTG
  GTCTTGGCCTCCAGAGGGAGGGCCTGGCTCACGGTGGGGCCAGGGAGCCGGCTCCAAAGGGTCTTCAAA
  AAGGGGGTCCTTGGGGGCTCCAGCTGCCTCGCCCTGGCCTTTCTGTGGGTGCGTGAGAGCCAGCAGCAC
  CCCAGCCTTGGAGACCGGGGGGGCAGGACCCCAAGTCCTCCCCTCTCTCCTGACTGCCCTGGCCGGGTG
  CCGGCACTGCGAGACCCACCTGGTGAGCAGGCCTCACAGTTCTTAGCCAGGGCCCCACCTCGCCTGTGT
  CCCACCAGTGCCCCGACAGACCTGGGGCAGGGCTGGGCCATGATGCAGCGGGCCAGGATAGCCTCCACC
  GTCAGCACAGGGCCGCCCTCCCCGCCTTTCCGGAGGAAACCACTCCCACCTCAGCCCAGCTGTGCGCCC
  TCCCTAGCTCTCCTGCCCCCTGGAGCTGATGGCCCCTTCTCCACTGACCGATTCCTTAGCGGGGCCTCT
  TGGGGTCTCGGGCCTCGGGTGCACCGTCCCATGCCCGTCCTGTTGTGGGCACCGTGGCCCTTGGGGCAG
  GCGGCTCTAATGCGGGAGCGAGTCCCTAGCTCCAGACTTAAGAACCAGACCCCGGGAGCATCTGGCATT
  TGGCGTGACGGCGTCGCAGGCGGGCCTGGGCTCCCTGGAGAGTGGCCTCCCTGGGAGTGAGCAGGGCTG
  GGGTCGTGGGCGCAAATACTCCTGCAGAGCAAGTGCAGGGGAGTCCTGGGCCCGTTTCTCCTCCACCTG
  CGTTTTCAGTGCACTTGGCTTGGCTGGGAGGTCCTGAGGCCCTGAGGCCAGCAGGGGAACCAGTCCTGA
  GGGAGAGGACTTTGAAAGCAGCATTTGAGGGTCGTACGCCCCTGGCTGGTGGGGGTCCTGGCGCTCAGG
  GTGTTCGGGGAGCCATGTCTGGCGTCCATTGTGGGGAGCTGCTGCCCTGGCCTCTCTGCCTACCCCCAG
  CCCGGCCAGGGCACTCCCAGGCCCTGTCGCCATTGAGGTGCCTCCGCTGGGCTGTCTCCTCACCCCTCC
  CTGTGCTGGAGCCTGTCCCAAAAAGGTGCCAACTGGGAGGCCTCGGAAGCCACTGTCCAGGCTCCCACT
  GCCTGTCTGCTCTGTTCCCAAAGGCAGCGTGTGTGGCCTCGGGCCCTGCGGTGGCATGAAGCATCCCTT
  CTGGTGTGGGCATCGCTACGTGTTTTGGGGGCAGCGTTTCACGGCGGTGCCCTTGCTGTCTCCCTTGG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_172108.5
• Summary: The M channel is a slowly activating and deactivating potassium channel that plays a critical role in the regulation of neuronal excitability. The M channel is formed by the association of the protein encoded by this gene and a related protein encoded by the KCNQ3 gene, both integral membrane proteins. M channel currents are inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 1 (BFNC), also known as epilepsy, benign neonatal type 1 (EBN1). At least five transcript variants encoding five different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 3785
• MW: 70.6