EPC1 (NM_025209) Human 3' UTR Clone

CAT#: SC201115

3' UTR clone of enhancer of polycomb homolog 1 (Drosophila) (EPC1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EPC1
• Synonyms: Epl1
• ACCN: NM_025209
• Insert Size: 1342 bp
• Sequence Data:
  Insert Sequence

  >SC201115 3’UTR clone of NM_025209
  The sequence shown below is from the reference sequence of NM_025209. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GACAACACAGTAGCGATGGAGGTGACGTAGCTTCCTCCGAGTGGAACTGCAGCCTGGGGACTTGATTAG
  GTGCTATGCATCAGTAGCATTTTGAATGCAAGGGATGATGGAAAGCAGCACATGCGTTTCTGTGGCAGC
  TGTGGGGACTTGACAGCAATGCTCATCTCTCATGCTTCAATTTTTCTTTTCTTACTGTTAATAGGAAAA
  AAATCAACATCTGTAAATTAAGAATACAGCAATTATCTGTACAGTACTGCATATTTGTAATACCCTTGT
  ATATATGTTACTTGAATACAGAAATGTTCATTTGTGATGCTTTGCACTTGGGGGTGGGGGGTGGGAGGG
  AAATCAATTGCAACAAAGAGTGTGAGATGTGAGATTGTATAGAGATGAAGTGTCATCACATCATGTGCA
  TGGTGCGGAACCTGCTGTTTTATCTATTTATTGTGCCGTGTTTACAGTTTTTTGTACACTGTACCTTCA
  TTGGTTCCTGTGCTGTAGTAAATGTGTTAGGTAGCTGTGGACTCCTTGGTATTTTGTAAATGGTATAAC
  ATAACTTGGTTCCCCTCTGGGTCCTTGAGTTTTCTGTGTATCATGTGAAAAAAAATGGTGACATACATA
  CAGAATTTTACAAAAAAAAAAAAAAAAAAAAAGGCATCAGTTTTTTTAAAAATGGGGAATGTACTATTA
  AATGGGGATCTTCCTGGTCTACTATCATTAGGACAAGTAACAAGCTAAAAATGAAGTCTCTTGAATCTT
  TCCATCCCCAACTTGCCCACAAAGCTGTGGGTGGTTTCTGGTACTTAAAGCACTAATATTATGTTGCTG
  CTCTGCGAACAGCCCAGTAGTCCCATTTCCTTCCACACCAAAAAGTGTTAAATCAAGTATGCAAATGGA
  GTCCTTACAACTGGAGTTTATGTTGTCTTGCCCTTTTTTTAGCACAAAAAAAATTGTACTTTTTTATTG
  TCGAATTGTTTAAAAGACTTCATTCTTTACTTGTTCTTACGAAAAGGATATAAGGGAGAAGAATGCAGT
  AATTGCTGTACGTGTATCATCATTCCAGTTTCTAAAAACAGCCAGCCAGCTTTTTTCCTTTTAAGATTC
  TCCAGAAGGCTGCAAGGCCAGAACCACAAATATCGGGTGCCTTCCTTTGGAAATATTTGACCTCTCTTC
  TGTGAAGAGAATGGTACTTAACAGACTACTACTAGTGCTTTGTCAGTACCGAAGTCTGAATGCCCAGTC
  CAATGGCATACATTATCCTTAAGGTTTTTAATCCCACCTGGAAAAATTGTGATAGAATTCTCACAAAAT
  AAACCCAGGGCATTACATGTTGACAAACTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_025209.5
• Summary: This gene encodes a member of the polycomb group (PcG) family. The encoded protein is a component of the NuA4 histone acetyltransferase complex and can act as both a transcriptional activator and repressor. The encoded protein has been linked to apoptosis, DNA repair, skeletal muscle differentiation, gene silencing, and adult T-cell leukemia/lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
• Locus ID: 80314
• MW: 50.1