Mouse IgG (H&L) Antibody Peroxidase Conjugated

CAT#: TA397852

Mouse IgG (H&L) Antibody Peroxidase Conjugated

Product Images

Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 30 min at RT. Predicted/Observed size: 50 kDa, 25 kDa for Mouse IgG. Other band(s): none.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1:2,000 - 1:10,000
  IHC: 1:500 - 1:2,500
  ELISA: 1:50,000 - 1:100,000
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
• Specificity: MOUSE IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
• Concentration: 2.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store HRP Conjugated Mouse Secondary Antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse HRP antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.