Fluorescein Conjugated Mouse IgG (H&L)

CAT#: TA397848

Fluorescein Conjugated Mouse IgG (H&L)

Product Images

Western Blot of Goat anti-Mouse IgG Fluorescein Conjugated Secondary Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: WB: User Optimized
  IF: 1:1,000 - 1:5,000
  FC: 1:500 - 1:2,500
  FLISA: 1:10,000 - 1:50,000
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule
• Specificity: Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: Anti-Mouse IgG FITC Conjugated Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.