Mouse IgG3 Antibody Peroxidase Conjugated Pre-adsorbed

CAT#: TA397825

Mouse IgG3 Antibody Peroxidase Conjugated Pre-adsorbed

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1:2,000 - 1:10,000
  IHC: 1:1,000 - 1:5,000
  ELISA: 1:20,000 - 1:100,000
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG3 heavy chain
• Specificity: Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: Anti-Mouse IgG3 peroxidase antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods and anti IgG2b antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types.