Mouse IgG1 Antibody Fluorescein Conjugated Pre-adsorbed

CAT#: TA397817

Mouse IgG1 Antibody Fluorescein Conjugated Pre-adsorbed

Product Images

Western Blot of Fluorescein conjugated Goat anti-Mouse IgG1 (Gamma 1 chain) Pre-adsorbed secondary antibody. Lane 1: Mouse IgG1. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): none.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:2,000 - 1:10,000
  IHC: 1:1,000 - 1:5,000
  IF: 1:1,000 - 1:5,000
  FC: 1:200 - 1:500
  FLISA: 1:10,000 - 1:50,000
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG heavy chain
• Specificity: Anti-Mouse IgG1 FITC conjugated secondary antibody prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: Mouse IgG1 FITC conjugated secondary antibody has been tested by western blot and is ideal for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.