Human IgG (H+L chain) Chicken Polyclonal Antibody
CAT#: R1332HRP
Human IgG (H+L chain) chicken polyclonal antibody, HRP
Conjugation: Unconjugated Biotin FITC
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunoblotting (1/1,000-1/5,000, Western or Dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. |
Reactivities | Human |
Host | Chicken |
Clonality | Polyclonal |
Immunogen | Human IgG whole molecule |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Human IgG and Human Serum. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Immunoaffinity Chromatography. |
Conjugation | HRP |
Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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