Goat IgG (H+L chain), adsorbed Donkey Polyclonal Antibody
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
Reactivities | Goat |
Host | Donkey |
Clonality | Polyclonal |
Immunogen | Goat IgG whole molecule. |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative Label: Texas Red State: Lyophilized purified Ig fraction. Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption emission: 596 nm / 620 nm Molar radio: 3.9 moles Texas Red per mole of Donkey IgG. |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Immunoaffinity chromatography. |
Conjugation | Texas Red |
Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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