SPINT1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4H2]

CAT#: TA504591AM

SPINT1 mouse monoclonal antibody, clone OTI4H2 (formerly 4H2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SPINT1 (Cat# RC218993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPINT1(Cat# TA504591). Positive lysates LY405735 (100ug) and LC405735 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPINT1 monoclonal antibody.

• 

  Anti-SPINT1 mouse monoclonal antibody (TA504591) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPINT1 (RC218993).

• 

  HEK293T cells transfected with either RC218993 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPINT1 antibody (TA504591), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4H2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:200, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SPINT1(NP_857593) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: serine peptidase inhibitor, Kunitz type 1
• Database Link:
  NP_857593
  Entrez Gene 20732 MouseEntrez Gene 311331 RatEntrez Gene 487501 DogEntrez Gene 704578 MonkeyEntrez Gene 6692 Human
  UniProt ID O43278
• Background: The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq]
• Synonyms: HAI; HAI1; MANSC2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane