ALG2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2A3]

CAT#: TA504217BM

ALG2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALG2 (RC204766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALG2. Positive lysates LY409737 (100ug) and LC409737 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALG2 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504217)

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  Anti-ALG2 mouse monoclonal antibody (TA504217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALG2 (RC204766).

Specifications

Product Data

• Clone Name: OTI2A3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALG2(NP_149078) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.9 kDa
• Gene Name: ALG2 alpha-1,3/1,6-mannosyltransferase
• Database Link:
  NP_149078
  Entrez Gene 85365 Human
  UniProt ID Q9H553
• Background: This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: CDGIi; hALPG2; NET38

Reference Data

• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis